Advertisement

Using Electrically Tunable Lens to Improve Axial Resolution and Imaging Field in Light Sheet Fluorescence Microscope

  • Muyue Zhai
  • Xiaoshuai Huang
  • Heng Mao
  • Qiudong Zhu
  • Shanshan WangEmail author
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 506)

Abstract

Light sheet fluorescence microscope (LSFM) has become one of the most promising 3D microscopy techniques due to its prominent lateral section illumination. In addition, comparing with the confocal microscope, it has low phototoxicity and high temporal resolution. In this paper, we proposed an easy-to-operate tiling light sheet fluorescence microscope, which integrates a cylindrical lens, a galvanometric mirror, and an excitation objective to produce a light sheet with desired numerical aperture and axial resolution and utilizes an electrically tunable lens (ETL) to enlarge the limited field of view (FOV) of single excitation section following the temporal multiplexing. Imaging performances have been characterized from the experiment by imaging the 20 nm fluorescent beads with water immersion, and the benefits of our proposed technique have been shown in volumetric resolution, FOV, and speed as well as contrast.

Keywords

Light sheet fluorescence microscope Electrically tunable lens Large field of view High axial resolution 

References

  1. 1.
    Huisken J, Swoger J, Del Bene F et al (2004) Optical sectioning deep inside live embryos by selective plane illumination microscopy[J]. Science 305(5686):1007–1009CrossRefGoogle Scholar
  2. 2.
    Ritter JG, Spille JH, Kaminski T et al (2011) A cylindrical zoom lens unit for adjustable optical sectioning in light sheet microscopy[J]. Biomed Opt Express 2(1):185–193CrossRefGoogle Scholar
  3. 3.
    Fu Q, Martin BL, Matus DQ et al (2016) Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy[J]. Nat Commun 7CrossRefGoogle Scholar
  4. 4.
    Chmielewski AK, Kyrsting A, Mahou P et al (2015) Fast imaging of live organisms with sculpted light sheets[J]. Sci Rep 5Google Scholar
  5. 5.
    Engelbrecht CJ, Stelzer EH (2006) Resolution enhancement in a light-sheet-based microscope (SPIM)[J]. Opt Lett 31(10):1477–1479CrossRefGoogle Scholar
  6. 6.
    Yan J. Principle and technology of laser[M]. Higher Education PressGoogle Scholar
  7. 7.
    Spring KR, Davidson MW. The depth of field and the depth of focus. https://www.microscopyu.com/microscopy-basics/depth-of-field-and-depth-of-focus

Copyright information

© Springer International Publishing AG, part of Springer Nature 2019

Authors and Affiliations

  • Muyue Zhai
    • 1
  • Xiaoshuai Huang
    • 2
  • Heng Mao
    • 3
  • Qiudong Zhu
    • 1
  • Shanshan Wang
    • 1
    Email author
  1. 1.Beijing Key Lab. for Precision Optoelectronic Measurement Instrument and TechnologySchool of Opto-Electronics, Beijing Institute of TechnologyBeijingChina
  2. 2.Institute of Molecular Medicine, Peking UniversityBeijingChina
  3. 3.LMAM, School of Mathematical Sciences, Peking UniversityBeijingChina

Personalised recommendations