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X-Ray Crystallography

  • Oksana Degtjarik
  • Gabriel Demo
  • Michaela Wimmerova
  • Ivana Kuta Smatanova
Chapter

Abstract

This chapter describes the fundamentals of the X-ray crystallography method and its application for studies of tertiary protein structures. A brief introduction to the theory of X-ray diffraction is given, followed by more detailed explanations of all protein structure determination stages, starting from crystal growth up to the final validation of protein structure. We emphasize the theoretical and practical aspects of the protein crystallization process which is the most crucial step of X-ray protein crystallography.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Oksana Degtjarik
    • 1
    • 2
  • Gabriel Demo
    • 3
    • 4
  • Michaela Wimmerova
    • 3
  • Ivana Kuta Smatanova
    • 2
    • 5
  1. 1.Department of Structural BiologyWeizmann Institute of ScienceRehovotIsrael
  2. 2.Institute of Chemistry and Biochemistry, University of South BohemiaČeské BudějoviceCzech Republic
  3. 3.CEITEC -Central European Institute of Technology, Masaryk UniversityBrnoCzech Republic
  4. 4.RNA Therapeutics Institute, University of Massachusetts Medical SchoolWorcesterUSA
  5. 5.Center for Nanobiology and Structural Biology of the Institute of MicrobiologyAcademy of Sciences of the Czech RepublicNove HradyCzech Republic

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