A 16-year-old African American male with sickle cell anemia is admitted to the hospital with worsening chest pain, shortness of breath, and fever (temperature 39.0 °C). Bilateral lower lobe infiltrates are seen on chest X-ray, and the patient’s oxygen saturation on room air is 88%. The patient’s hemoglobin (Hgb) level is 6.2 g/dL (his baseline Hgb is 7.5 g/dL). The patient has not been seen in your hospital prior to this admission, but the patient does report a history of blood transfusions, including several units of red blood cells (RBCs) transfused 2 months ago for severe pain while traveling abroad. An ethylenediaminetetraacetic acid (EDTA) anticoagulant sample is submitted to the blood bank for type and screen.
KeywordsDNA-based testing Genotyping Massively parallel sequencing Next-generation sequencing Polymerase chain reaction Sickle cell anemia Single nucleotide polymorphisms
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