• Juntaro Kato
  • Mayuko Inari-Ikeda
  • Mai Hayashi
  • Junji Amano
  • Hiroaki Ohashi
  • Masahiro MiiEmail author
Part of the Handbook of Plant Breeding book series (HBPB, volume 11)


Approximately 500 species are involved in the genus Primula (Primulaceae). Most of these species are found in cool regions, high-altitude mountain area, or high-latitude area, in northern hemisphere. Interspecific hybridizations were performed among several species of the genus Primula to extend variations, to combine unique traits of both parents, and to introduce useful traits of one species into the other species. However, the abortion of hybrid embryos was one of the serious barriers for interspecific hybridizations. By using the appropriate medium developed in our study, interspecific hybrids were successfully produced in several interspecific cross combinations. Although all of the diploid hybrids obtained in these crosses showed hybrid sterility, female fertility was found to some extent in triploid hybrids. These triploids resulted in some interspecific cross combinations from the fertilizations between spontaneous unreduced gametes and haploid ones. Some of the hybrids or BC1s expressed attractive or unique characters and might be used as novel cultivars directly or as useful pre-breeding material for further breeding.


Embryo rescue Hybrid sterility Interspecific hybridization Primula Unreduced gamete Unreduced 3x gamete Polyploid 


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Juntaro Kato
    • 1
  • Mayuko Inari-Ikeda
    • 2
  • Mai Hayashi
    • 3
  • Junji Amano
    • 3
  • Hiroaki Ohashi
    • 4
  • Masahiro Mii
    • 3
    • 5
    Email author
  1. 1.Department of Biology, Aichi University of EducationKariyaJapan
  2. 2.Department of Nutrition, School of Health and NutritionTokai Gakuen UniversityNagoyaJapan
  3. 3.Laboratory of Plant Cell Technology, Graduate School of HorticultureChiba UniversityMatsudoJapan
  4. 4.Faculty of Agriculture, Ehime UniversityMatsuyamaJapan
  5. 5.Center for Environment, Health and Field SciencesChiba UniversityKashiwaJapan

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