• Chia-Chi Hsu
  • Hong-Hwa Chen
  • Wen-Huei Chen
Part of the Handbook of Plant Breeding book series (HBPB, volume 11)


Phalaenopsis is one of the most popular cultivated orchids worldwide. So far, 92 native species and 34,112 hybrids of Phalaenopsis have been registered in the Royal Horticultural Society (RHS), but only 18 native species are frequently used for breeding. In Phalaenopsis market, large flowers with various colors are most popular. Phal. amabilis and Phal. aphrodite are the major species used for breeding the white-and-large-flower hybrids. The colored hybrids include pink, red-to-purple, green, yellow-to-orange, and black colors, as well as distinct colors in tepals with red lip. For large-and-red flowers, Phal. schilleriana and Phal. sanderiana are the major parent species, whereas Phal. equestris and Phal. pulcherrima are important for the development of the small- and medium-sized red flowers. Members of the subgenus Polychilos are the most important ancestors for yellow-to-orange flowers. More recently, there is an increased interest in plants with white, pink, or yellow colors with red stripes and/or spots. These traits are introduced from Phal. lindenii, Phal. stuartiana, and Phal. amboinensis. Furthermore, harlequin flowers which have clown-like spots and very complicated color patterns are released in the market, as well as hybrids with peloric and Bigfoot flowers. Besides color, scent and (a)biotic stress resistance are becoming important targets in Phalaenopsis breeding.


Bigfoot Breeding Harlequin Orchids Peloric Phalaenopsis 


  1. Arends JC (1970) Cytological observations on genome homology in eight interspecific hybrids of Phalaenopsis. Gentica 41:88–100CrossRefGoogle Scholar
  2. Borevitz JO, Xia Y, Blount J, Dixon RA, Lamb C (2000) Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis. Plant Cell 12:2383–2393CrossRefPubMedPubMedCentralGoogle Scholar
  3. Butelli E, Licciardello C, Zhang Y, Liu J, Mackay S, Bailey P, Reforgiato-Recupero G, Martin C (2012) Retrotransposons control fruit-specific, cold-dependent accumulation of anthocyanins in blood oranges. Plant Cell 24(3):1242–1255CrossRefPubMedPubMedCentralGoogle Scholar
  4. Chen WH (2017) The development of Phalaenopsis orchid industry in Taiwan. Orchid Digest 81-1:22–27Google Scholar
  5. Chen WH, Hsu CY, Cheng HY, Chang H, Chen HH, Ger MJ (2011) Downregulation of putative UDP-glucose: flavonoid 3-O-glucosyltransferase gene alters flower coloring in Phalaenopsis. Plant Cell Rep 30:1007–1017CrossRefPubMedGoogle Scholar
  6. Chen WH, Kao YK, Tang CY (2014) Variation of the Genome Size among Phalaenospsis species using DAPI. J Taiwan Soc Hortic Sci 60:115–123Google Scholar
  7. Chen WH, Kao YL, Tang CH (2013a) Method for producing polyploid plants of orchids. US Patent 8,383,881Google Scholar
  8. Chen WH, Kao YK, Tang CY, Tsai CC, Lin TY (2013b) Estimating nuclear DNA content within 50 species of the genus Phalaenopsis Blume (Orchidaceae). Sci Hortic (Amsterdam) 161:70–75CrossRefGoogle Scholar
  9. Chiu LW, Zhou X, Burke S, Wu X, Prior RL, Li L (2010) The purple cauliflower arises from activation of a MYB transcription factor. Plant Physiol 154(3):1470–1480CrossRefPubMedPubMedCentralGoogle Scholar
  10. Christenson EA (2001) Phalaenopsis. In: Phalaenopsis: a monograph. Timber Press, PortlandGoogle Scholar
  11. Chuang HT, Hsu ST, Shen TM (2008) Breeding barriers in yellow Phalaenopsis orchids. J Taiwan Soc Hortic Sci 54:59–66Google Scholar
  12. Darwin C (1868) The variation of animals and plants under domestication, 1st edn. John Murray, LondonGoogle Scholar
  13. Feller A, Machemer K, Braun EL, Grotewold E (2011) Evolutionary and comparative analysis of MYB and bHLH plant transcription factors. Plant J 66:94–116CrossRefPubMedGoogle Scholar
  14. Griesbach RJ (1985) Polyploidy in Phalaenopsis orchid improvement. J Hered 76:74–75CrossRefGoogle Scholar
  15. Griesbach RJ (2005) A scientific approach to breeding blue orchid-exploring new frontiers in search of elusive flower colors. Orchids 74:376–379Google Scholar
  16. Grotewold E (2006) The genetics and biochemistry of floral pigments. Annu Rev Plant Biol 57:761–780CrossRefPubMedGoogle Scholar
  17. Hichri I, Barrieu F, Bogs J, Kappel C, Delrot S, Lauvergeat V (2011) Recent advances in the transcriptional regulation of the flavonoid biosynthetic pathway. J Exp Bot 62:2465–2483CrossRefPubMedGoogle Scholar
  18. Hsiao YY, Jeng MF, Tsai WC, Chuang YC, Li CY, Wu TS, Kuoh CS, Chen WH, Chen HH (2008) A novel homodimeric geranyl diphosphate synthase from the orchid Phalaenopsis bellina lacking a DD(X)2-4D motif. Plant J 55:719–733CrossRefPubMedGoogle Scholar
  19. Hsiao YY, Tsai WC, Kuoh CS, Huang TH, Wang HC, Wu TS, Leu YL, Chen WH, Chen HH (2006) Comparison of transcripts in Phalaenopsis bellina and Phalaenopsis equestris (Orchidaceae) flowers to deduce monoterpene biosynthesis pathway. BMC Plant Biol 6:14CrossRefPubMedPubMedCentralGoogle Scholar
  20. Hsu CC, Chen WH (2015) The breeding achievements from Phalaenopsis equestris. Malayan Orchid Rev 49:41–47Google Scholar
  21. Hsu CC, Chen YY, Tsai WC, Chen WH, Chen HH (2015a) Three R2R3-MYB transcription factors regulate distinct floral pigmentation patterning in Phalaenopsis spp. Plant Physiol 168:175–191CrossRefPubMedPubMedCentralGoogle Scholar
  22. Hsu HF, Hsu WH, Lee YI, Mao WT, Yang JY, Li JY, Yang CH (2015b) Model for perianth formation in orchids. Nature Plants 1:15046CrossRefGoogle Scholar
  23. Kao YY, Chiang SB, Lin TY, Hsieh CH, Chen YH, Chen WH, Chen CC (2001) Differential accumulation of heterochromatin as a cause for karyotype variation in Phalaenopsis orchids. Ann Bot 87:387–395CrossRefGoogle Scholar
  24. Koes R, Verweij W, Quattrocchio F (2005) Flavonoids: a colourful model for the regulation and evolution of biochemical pathways. Trends Plant Sci 10:236–242CrossRefPubMedGoogle Scholar
  25. Kuo PC, Wu TS (2011) Biosynthetic pathway of pigments in Phalaenopsis species. Orchid Biotechnol II:129–144CrossRefGoogle Scholar
  26. Lin S, Lee H, Chen W, Chen C, Kao Y, Fu Y, Chen Y, Lin T (2001) Nuclear DNA contents of Phalaenopsis sp. and Doritis pulcherrima. J Am Soc Hortic Sci 126:195–199Google Scholar
  27. Ma H, Pooler M, Griesbach R (2009) Anthocyanin regulatory/structural gene expression in Phalaenopsis. J Am Soc Hortic Sci 134:88–96Google Scholar
  28. OrchidWiz (2017) OrchidWiz Encyclopedia Version X3.3 May 2017 Database. OrchidWiz Database LLC, AmesGoogle Scholar
  29. Petroni K, Tonelli C (2011) Recent advances on the regulation of anthocyanin synthesis in reproductive organs. Plant Sci 181:219–229CrossRefPubMedGoogle Scholar
  30. Sasaki N, Nakayama T (2015) Achievements and perspectives in biochemistry concerning anthocyanin modification for blue flower coloration. Plant Cell Physiol 56:28–40CrossRefPubMedGoogle Scholar
  31. Sattler MC, Carvalho CR, Clarinde WR (2016) The polyploidy and its role in plant breeding. Planta 243:281–296CrossRefPubMedGoogle Scholar
  32. Shindo S, Kamemoto H (1963) Karyotype analysis of some species of Phalaenopsis. Cytologia 28:390–398CrossRefGoogle Scholar
  33. Su V, Hsu BD (2003) Cloning and expression of a putative cytochrome P450 gene that influences the colour of Phalaenopsis flowers. Biotechnol Lett 25:1933–1939CrossRefPubMedGoogle Scholar
  34. Tanaka Y, Ohmiya A (2008) Seeing is believing: engineering anthocyanin and carotenoid biosynthetic pathways. Curr Opin Biotechnol 19:190–197CrossRefPubMedGoogle Scholar
  35. Tsai WC, Kuoh CS, Chuang MH, Chen WH, Chen HH (2004) Four DEF-like MADS box genes displayed distinct floral morphogenetic roles in Phalaenopsis orchid. Plant Cell Physiol 45:831–844CrossRefPubMedGoogle Scholar
  36. Wang J, Ming F, Han Y, Shen D (2006) Flavonoid-3′,5′-hydroxylase from Phalaenopsis: a novel member of cytochrome P450s, its cDNA cloning, endogenous expression and molecular modeling. Biotechnol Lett 28:327–334CrossRefPubMedGoogle Scholar
  37. Winkel-Shirley B (2001) Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiol 126:485–493CrossRefPubMedPubMedCentralGoogle Scholar
  38. Yeh YC, Tsai YS, Shih CT, Huang P (2012) The breeding of scented flowers in Phalaenopsis. In: Orchid breeding in Taiwan. Published by Taiwan Orchid Breeders Society. pp 86–94Google Scholar
  39. Zhang G, Lu S, Chen TA, Funk CR, Meyer WA (2003) Transformation of triploid bermuda grass (Cynodon dactylon × C. transvaalensis cv. TifEagle) by means of biolistic bombardment. Plant Cell Rep 21:860–864PubMedGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Chia-Chi Hsu
    • 1
  • Hong-Hwa Chen
    • 1
    • 2
  • Wen-Huei Chen
    • 2
  1. 1.Department of Life SciencesNational Cheng Kung UniversityTainanTaiwan
  2. 2.Orchid Research and Development CenterNational Cheng Kung UniversityTainanTaiwan

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