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DNA Barcoding on Bacteria and Its Application in Infection Management

  • Mohammad Zubair
  • Farha Fatima
  • Shamina Begum
  • Zahid Hameed Siddiqui
Chapter

Abstract

The development of bacterial DNA barcoding with error-independent and time-saving technique has increased dramatically, which helps in identifying the microbes, understanding the microbial biodiversity, and analyzing the diseases, related to these pathogens. The study has discussed the developmental stages of DNA barcode research and compared it with the gold standard methods. It has evaluated the possible application of magnetic- and nanoparticle-related applications in bacterial DNA barcoding. These innovative techniques help in the identification of a number of infectious pathogens, which are present in trace amount. Bio-imaging detection of an infectious microorganism has proved to be effective for the development of fluorescent nanoparticle, super-paramagnetic nanoparticle, and metallic nanoparticle. The living organisms, present with functional materials, have a vast application in clinical research of water-soluble conjugated polymers. However, the performance of DNA barcoding in laboratory scale is easy, and it tends to give maximum benefit to the community, if they developed a global system to share their findings and interpret the sequenced data by genome.

Keywords

Bacteria DNA barcoding Infection management 

Abbreviations

Bg

Bacillus globigii

BLAST

Basic local alignment search tool

BOLD

The barcode of life data system

CPNs

Conjugated polymer nanoparticles

CTX

Cefotaximases

E. coli

Escherichia coli

FRET

Fluorescence resonance energy transfer

kDa

Kilo daltons

cpn60

Chaperonin 60

MLST

Multilocus sequence typing

MOTU

Molecular operational taxonomic unit

MTB

Mycobacterium tuberculosis

NCBI

National Centre for Biotechnology Information

mecA

Novel penicillin-binding protein, PBP-2a

NPs

Nanoparticles

NTM

Nontuberculous mycobacteria

PAIs

Potential pathogenicity islands

PCR

Polymerase chain reaction

QBOL

The quarantine barcoding of life

RIF

Replication initiation factor

S. aureus

Staphylococcus aureus

SHV

Sulfhydryl variable enzymes

Tuf

Elongation factor Tu

Notes

Acknowledgment

The authors are very thankful to all the associated personnel in any reference that contributed in/for the purpose of this research.

Conflict of Interest

The authors declare no conflict of interest.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Mohammad Zubair
    • 1
  • Farha Fatima
    • 2
  • Shamina Begum
    • 1
  • Zahid Hameed Siddiqui
    • 3
  1. 1.Department of Medical Microbiology, Faculty of MedicineUniversity of TabukTabukKingdom of Saudi Arabia
  2. 2.Department of Zoology, Faculty of Life ScienceAligarh Muslim UniversityAligarhIndia
  3. 3.Department of Biology, Faculty of ScienceUniversity of TabukTabukKingdom of Saudi Arabia

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