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A Molecular Assessment of Red Algae with Reference to the Utility of DNA Barcoding

  • Zahid Hameed Siddiqui
  • Zahid Khorshid Abbas
  • Khalid Rehman Hakeem
  • Mather Ali Khan
  • Abdul Ilah
Chapter

Abstract

The ecological and commercial importance of red algae is of high value, the estimated cost seaweed industry produces is US$10 billion. The species which are exploited most are the members of Rhodophyta (Eucheuma/Kappaphycus, Porphyra, and Gracilaria). In order to understand the distribution of seaweed, their identification is necessary which is generally based on morphological characteristics, often resulting in wrong identification of species. DNA barcoding can be used as a contemporary tool for species identification. It can resolve many intrinsic problems of morphological taxonomy, only a small amount of tissue is required for species identification, and the samples can be examined at all stages of development. The application of DNA barcoding can be used in identification of invasive and endangered species along with conservation biology. In case of red alga, DNA barcoding proved to be beneficial for the recognition of high-yielding agar strain as well as for cryptic species identification. In this study, several identification-based problems of red algae have been discussed by using different intraspecific markers such as cox1, cox3, and cox2-3 spacer and rbcL and rbcL-rbcS spacer. As per the available data, the mitochondrial markers, gene cox1 is more effective than rbcL for the measurement of red algal genetic diversity.

Keywords

DNA barcoding Red algae Cox1 Cox2-3 rbcL 

Abbreviations

AFLP

Amplified fragment length polymorphism

BOLD

Barcode of Life Database

CBOL

Consortium for the Barcode of Life

cox1

Mitochondrial cytochrome oxidase subunit 1

cox2

Mitochondrial cytochrome oxidase subunit 2

cox3

Mitochondrial cytochrome oxidase subunit 3

DNA

Deoxyribonucleic acid

ISSR

Inter-simple sequence repeats

ITS-1 and ITS-2

The internal transcribed spacers

RAPD

Random amplified polymorphic DNA

rbcL

Large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo)

rbcL-rbcS

The plastid-encoded RuBisCO spacer

rDNA (SSU rDNA)

The nuclear small subunit

RFLP

Restriction fragment length polymorphism

Notes

Conflict of Interest

The authors declare that there is no conflict of interest.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Zahid Hameed Siddiqui
    • 1
  • Zahid Khorshid Abbas
    • 1
  • Khalid Rehman Hakeem
    • 2
  • Mather Ali Khan
    • 3
  • Abdul Ilah
    • 4
  1. 1.Department of Biology, Faculty of ScienceUniversity of TabukTabukKingdom of Saudi Arabia
  2. 2.Department of Biological Sciences, Faculty of ScienceKing Abdulaziz UniversityJeddahKingdom of Saudi Arabia
  3. 3.Bond Life Sciences Center, University of Missouri-ColumbiaColumbiaUSA
  4. 4.Faculty of Medical TechnologyOmar Al Mukhtar UniversityAl BaydaLibya

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