Metallomics pp 111-137 | Cite as

The Use of Stable Isotopic Tracers in Metallomics Studies

  • Maria Montes-BayónEmail author
  • Jörg Bettmer
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1055)


Mass spectrometry represents an essential technique in Metallomics studies. It permits the identification of metal-containing molecules as part of the metallome as well as their quantification at low concentration levels. The technique becomes even more powerful in combination with the use of isotopically enriched species. Provided that they are stable, these isotopically labelled species can be easily distinguished from their natural counterparts by mass spectrometric techniques. This capability permits that these species are used for accurate and precise quantitative experiments and/or metabolic studies applying inductively coupled plasma as ionization source. In this chapter, we present the different concepts of using stable isotope tracers and isotope dilution analysis as quantification strategy. Besides some fundamental aspects, various examples from Metallomics studies, for instance, on the preparation of isotopically enriched metalloproteins and determination by isotope dilution analysis or the exploration of the biological pathways of Se species, are shown in order to demonstrate the usefulness of isotopes.


Isotopes ICP-MS Isotope dilution analysis Tracer studies Metalloproteins Metallometabolites 



Absolute quantification


Certified reference material


Deoxyribonucleic acid


Dissolved organic matter


Electrospray ionization


Fractional absorption of zinc


Gas chromatography-mass spectrometry


High-performance liquid chromatography


Inductively coupled plasma


Inductively coupled plasma-mass spectrometry


Isotope dilution analysis


Isotope pattern deconvolution


Laser ablation-inductively coupled plasma-mass spectrometry


Liquid chromatography-mass spectrometry


Liquid chromatography-tandem mass spectrometry


Matrix-assisted laser desorption and ionization


Multicollector-inductively coupled plasma-mass spectrometry


Model for end-stage liver disease


Se-methyl selenocysteine


Mass spectrometry


Negative thermal ionization-mass spectrometry


Polymerase chain reaction


Relative standard deviation






Superoxide dismutase


Thermal ionization mass spectrometry


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.University of Oviedo, Department of Physical and Analytical ChemistryOviedoSpain

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