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Metallomics pp 101-110 | Cite as

Metallomics in Fish

  • Camila Pereira Braga
  • Jiri Adamec
  • Pedro de Magalhães Padilha
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1055)

Abstract

Metallomics allows the integration of traditionally analytical studies with inorganic and biochemical studies. The study of metallomics in living organisms allows us to obtain information about how the metal ion is distributed and coordinated with proteins, the essentiality and/or toxicity, and the individual concentrations of metal species, thus contributing to elucidation of the physiological and functional aspects of these biomolecules. In this context, several lines of research have appeared in the literature with different terms and approaches. For example, metallomic, which deals with the characterization of the total metal/metalloid species present in an organism; metalloprotein, which deals with the characterization of the total elements present in a specific site of an organism (cellular behavior, protein, metalloprotein); and metallomic, which deals with a more in-depth study of metallome. In this area, information is sought on the interactions and functional connections of metal/metalloid species with genes, proteins, metabolites and other biomolecules of the organism and, therefore, the elucidation of the biological role exerted by the metal ions bound to the biomolecules. In this chapter, we will describe techniques used in animal studies.

Keywords

Animal Fish Metallomic Metalloprotein 

Abbreviations

2D-PAGE

Two-dimensional polyacrylamide gel electrophoresis

AAS

Atomic absorption spectrometry

ESI

Electrospray ionization

ESI-MS/MS

Electrospray ionization tandem mass spectrometry

FAAS

Flame atomic absorption spectrometry

GFAAS

Graphite furnace atomic absorption spectrometry

ICP-MS

Inductively coupled plasma source mass spectrometry

IEF

Isoelectric focusing

LC

Multidimensional liquid chromatography

MALDI

Matrix assisted laser desorption ionization

MALDI-TOF /MS

Time-of-flight mass spectrometry coupled to laser-assisted matrix

Mm

Molecular mass

MS

Mass spectrometry

PAGE

One-dimensional polyacrylamide gel electrophoresis

pI

Isoeletric point

SDS-PAGE

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

SEC

size-exclusion chromatography

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Camila Pereira Braga
    • 1
  • Jiri Adamec
    • 1
  • Pedro de Magalhães Padilha
    • 2
  1. 1.Department of BiochemistryUniversity of Nebraska-LincolnLincolnUSA
  2. 2.Department of Chemistry and BiochemistryInstitute of Bioscience, São Paulo State University (UNESP)BotucatuBrazil

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