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Genotyping of Festulolium Cultivars Involved in EUCARPIA Multi-site Trial Using DArT Markers and GISH

  • D. KopeckýEmail author
  • J. Baert
  • S. Barth
  • J. Bartoš
  • V. Černoch
  • J. Doležel
  • D. Grogan
  • J. Harper
  • M. Humphreys
  • T. Książczyk
  • L. Østrem
  • E. Paszkowski
  • D. Sokolovič
  • Z. Zwierzykowski
  • M. Ghesquière
Conference paper

Abstract

A comprehensive set of Festulolium cultivars from on-going field trials in the Eucarpia network was characterised at the chromosome level using genomic in situ hybridization (GISH) and by Diversity Array Technology (DArT) markers. Both technologies were found to be complementary in describing the breeding history of the plant material. The genomic composition of the Lolium × Festuca cultivars varied from those that comprised equivalent proportions of their parental genomes to introgression lines where small chromosome segments of Festuca had been translocated onto Lolium chromosomes. The breadth of genotype combinations found within the grass cultivars described represents an important resource of genetic variations necessary to combat the diverse abiotic stresses encountered within Europe, including safeguards against prolonged exposure to harsh weather conditions. It is likely that in future plant breeding, genotyping will contribute to precision-transfers of targeted Festuca genes into Lolium germplasm in order to enhance resilience to climate change.

Keywords

Festulolium Interspecific hybridization Genotyping Genetic variability Genome composition 

Notes

Acknowledgements

DK, JB and JD were supported by the National Program of Sustainability I (award LO1204).

References

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • D. Kopecký
    • 1
    Email author
  • J. Baert
    • 2
  • S. Barth
    • 3
  • J. Bartoš
    • 1
  • V. Černoch
    • 4
  • J. Doležel
    • 1
  • D. Grogan
    • 5
  • J. Harper
    • 6
  • M. Humphreys
    • 6
  • T. Książczyk
    • 7
  • L. Østrem
    • 8
  • E. Paszkowski
    • 9
  • D. Sokolovič
    • 10
  • Z. Zwierzykowski
    • 7
  • M. Ghesquière
    • 11
  1. 1.Institute of Experimental BotanyCentre of the Region Hana for Biotechnological and Agricultural ResearchOlomoucCzech Republic
  2. 2.ILVOMelleBelgium
  3. 3.Oak Park Crops Research CentreTeagascCarlowIreland
  4. 4.DLF Seeds, s.r.o.Hladké ŽivoticeCzech Republic
  5. 5.Department of Agriculture, Food and MarineTipperaryIreland
  6. 6.IBERSAberystwyth UniversityAberystwythUK
  7. 7.Institute of Plant GeneticsPoznanPoland
  8. 8.NIBIO, FurenesetHellevik i FjalerNorway
  9. 9.DANKO Plant Breeding Ltd.PiaskiPoland
  10. 10.Institute of Forage CropsGloboder, KruševacSerbia
  11. 11.INRALusignanFrance

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