Rough Lemon (Citrus Jambhiri Lush.)

  • Savita
  • Pratap Kumar Pati
  • Avinash Kaur NagpalEmail author
Part of the Forestry Sciences book series (FOSC, volume 85)


The genus Citrus is an important group of fruit crops worldwide; belongs to family Rutaceae comprising 140 genera and 1300 species distributed throughout the world. Some of the well known fruits of citrus group are oranges, lemons, grapefruits and limes.


  1. Altaf N, Khan AR, Ali L, Bhatti IA (2008) Propagation of rough lemon (Citrus jambhiriLush.) through in vitro culture and adventitious rooting in cuttings. Elect J Environ Agric Food Chem 7:3326–3333Google Scholar
  2. Bhattacharya SC, Dutta S (1952) A preliminary observation on rootstocks for Citrus in Assam. Indian J Horticulture 9:1–11Google Scholar
  3. Bitters WP, Murashige T, Rangan TS, Nauer E (1970) Investigations on established virus-free plants through tissue culture. California Citrus Nursery Soc 9:27–30Google Scholar
  4. Carimi F, De Pasquale F, Crescimanno FG (1999) Somatic embryogenesis and plant regeneration from pistil thin cell layers of Citrus. Plant Cell Rep 18:935–940CrossRefGoogle Scholar
  5. Carimi F, Tortorici MC, De Pasquale F’, Somatic GF (1998) embryogenesis and plant regeneration from undeveloped ovules and stigma/style explant of sweet orange navel group [Citrus sinensis (L.) Qsb.]. Plant Cell Tissue Organ Culture 54:183–189Google Scholar
  6. Chaturvedi HC, Singh SK, Sharma AK, Agnihotri S (2001) Citrus tissue culture employing vegetative explants. Indian J Exp Biol 39:1080–1095PubMedGoogle Scholar
  7. D’Onghia AM, Carimi F, De Pasquale F, Djelouah K, Martelli GP (2001) Somatic embryogenesis from style culture: a new technique for the sanitation, conservation and safe exchange of citrus germplasm. In: Proceedings ix international society of citricultureGoogle Scholar
  8. Duan YX, Liu X, Fan J, Li DL, Wu RC, Guo WW (2007) Multiple shoot induction from seedling epicotyls and transgenic citrus plant regeneration containing the green fluorescent protein gene. Botanical Stud 48:165–171Google Scholar
  9. Fridborg G, Pedersen M, Landstrom LE, Eriksson T (1978) Effects of activated charcoal on tissue cultures: adsorption of metabolites in plant tissue cultures. Physiol Plant 43:104–106CrossRefGoogle Scholar
  10. Grosser JW, Gmitter FG Jr, Chandler JL (1988) Intergeneric somatic hybrid plants of Citrus sinensis CV. Hamlin and Poncirustrifoliata CV. Flying Dragon. Plant Cell Rep 7:5–8CrossRefPubMedGoogle Scholar
  11. Guo WW, Grosser JW (2005) Somatic hybrid vigor in Citrus: Direct evidence from protoplast fusion of an embryogenic callus line with a transgenic mesophyll parent expressing the GFP gene. Plant Sci 168:1541–1545CrossRefGoogle Scholar
  12. Iyer RI, Jayaraman G, Ramesh A (2009) Direct somatic embryogenesis in Myristicamalabarica Lam., an endemic, threatened medicinal species of Southern India and detection of phytochemicals of potential medicinal value. Indian J Sci Technol 7:11–17Google Scholar
  13. Madhav MS, Rao RN, Singh S, Deka PC (2002) Nucellar embryogenesis and artificial seed production in Citrus reticulata. Plant Cell Biotechn Molecular Biology 3:77–80Google Scholar
  14. Miah MN, Islam S, Hadiuzzaman S (2002) Regeneration of plantlets through somatic embryogenesis from nucellus tissue of CitrusmacropteraMont. var. anammensis(‘Sat Kara’). Plant TissueCulture 12:167–172Google Scholar
  15. Moiseeva NA, Serebryakova VN, Nardi L, Lucretti S, Butenko RG (2006) Organization of initial stages of somatic embryogenesis in tissue culture of Citrus sinensis cv. Tarocco at the organismal level. Russian J Plant Physiol 53:548–555CrossRefGoogle Scholar
  16. Mukhtar R, Khan MM, Fatima B, Abbas M, Shahid A (2005) In vitro regeneration and multiple shoots induction in Citrus reticulata Blanco. Int J Agric Biology 7:414–416Google Scholar
  17. Pérez RM, Mas O, Navarro L, Duran-Vila N (1999) Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrid. Plant Cell Tissue and Organ Culture 55:71–74Google Scholar
  18. Rangacharlu VS, Naidu N (1958) Anjaneyulu; Narsimharao, T.L. Performance of Sathgudi orange (C.sinensisOsbeck) on different rootstocks at Kodur (South India). Indian J Horticulture 15:35–58Google Scholar
  19. Rangan TS, Murashige T, Bitters WP (1968) In vitro initiation of nucellar embryos in monoembryonate Citrus. Hort Sci 3:226–227Google Scholar
  20. Redenbaugh K, Fujii J, Slade D (1988) Encapsulated plant embryos. In: Green C, Somers D, Hackett W, Biesboer D (eds) Advances in biotechnological processes, vol 9. Liss, New York, pp 473–493Google Scholar
  21. Ricci AP, Filho MFAA, Mendes BMJ, Piedade SM (2002) Somatic embryogenesis in Citrus sinensis, C.reticulataand C. nobilis × C. deliciosa. Scientia Agricola 59:41–46CrossRefGoogle Scholar
  22. Saunt J (1990) Citrus varieties of the world. Sinclair International Ltd.Google Scholar
  23. Savita, Pati PK, Nagpal A, Virk GS (2015) An somatic embryogenesis protocol for Citrus jambhiri and assessment of clonal fidelity of plantlets using RAPD markers. J Plant Growth Regulation 34(2):309–319Google Scholar
  24. Savita, Singh B, Virk GS, Nagpal A (2011) Efficient micropropagation protocol for regeneration of Citrus jambhiri Lush.using cotyledon as explant. Physiol Molecular Biology Plants 17:161–169Google Scholar
  25. Singh B, Sharma S, Rani G, Zaidi AA, Virk GS, Nagpal A (2006) In vitro flowering in embryogenic cultures of kinnow mandarin (Citrus nobilisLour × C. deliciosaTenora). Afr J Biotech 5:1470–1474Google Scholar
  26. Singh LB (1962) Studies on the rootstocks for sweet orange in the web tropics I Variety Rangtra. Hort Adv 6:19–27Google Scholar
  27. Singh S, Rajam MV (2009) Citrus biotechnology: Achievements, limitations and future directions. Physiol Molecular Biology Plants 15:3–22CrossRefGoogle Scholar
  28. Siragusa M, Carra A, Salvia L, Puglia AM, De Pasquale F, Carimi F (2007) Genetic instability in calamondin (Citrus madurensisLour.) plants derived from somatic embryogenesis induced by diphenylurea derivatives. Plant Cell Reports 26:1289–1296CrossRefPubMedGoogle Scholar
  29. Talon M, Gmitter GF Jr (2008) Citrus Genomics. Int J Plant Genomics 1–17:2008Google Scholar
  30. Wu J-H, Mooney P (2002) Autotetraploidtangor plant regeneration from in vitro Citrus somatic embryogenic callus treated with colchicine. Plant Cell Tissue Organ Cult 70:99–104Google Scholar
  31. Yang XL, Kitajima A, Hasegawa K (2000) Callus induction and embryoid regeneration from the endosperm culture of ‘Tosa-Buntan’ pummelo (Citrus grandis[L.] Osb.). Environ Control Biology 38:241–246. (in Japanese)CrossRefGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Savita
    • 1
  • Pratap Kumar Pati
    • 2
  • Avinash Kaur Nagpal
    • 3
    Email author
  1. 1.Botany Department, Hindu CollegeUniversity of DelhiNew DelhiIndia
  2. 2.Department of BiotechnologyGuru Nanak Dev UniversityAmritsarIndia
  3. 3.Department of Botanical and Environmental SciencesGuru Nanak Dev UniversityAmritsarIndia

Personalised recommendations