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Somatic Embryogenesis in Guava (Psidium guajava L.)

  • Nasim Akhtar
Chapter
Part of the Forestry Sciences book series (FOSC, volume 85)

Abstract

Guava (Psidium guajava L.) is well known for edible fruit rich in proteins, carbohydrates, minerals, sugars, oils and vitamin-C. All parts of the plant pharmaceutical used as anti-diarrhoeal, antimicrobial, antimalarial, antitussive, antioxidant, antigenotoxic and antimutagenic agents. Seed cultivation displaying heterozygous gene pool with genetic variability in both plant and fruit characteristics. The conventional propagation and in vitro organogenesis do not meet the demand for the good quality planting materials. Somatic embryogenesis for efficient micropropagation of guava (Psidium guajava L.) has been developed to fillup the gap. Somatic embryogenesis and plantlets regeneration are achieved from 10-week post anthesis zygotic embryo explants by 8-day inductive treatment with different concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D) on MS agar medium containing 5% sucrose. Subsequent development and maturation of somatic embryos occur after 8 days on MS basal medium supplemented with 5% sucrose without plant growth regulator. The process of somatic embryogenesis has shown the highest frequency (85.58%), intensity (360.04 embryos per culture), as well as moderate frequency of convertible elongated and short torpedo stage somatic embryos (16.17%, 36.95%, respectively) hence, the highest relative efficiency (167.72) in 8-day treatment of zygotic embryo explants with 1.0 mg l–1 2,4-D. High efficiency germination of somatic embryos and plantlet regeneration take place on half strength semi-solid MS medium amended with 3% sucrose within 2 weeks of subculture. Somatic plantlets are grown for additional 2 weeks by subculturing in MS liquid growth medium containing 3% sucrose. Well-grown plantlets from liquid medium have survived very well following 2–4 week hardening process. The protocol of somatic embryogenesis is optimized for high efficiency micropropagation of guava species.

Keywords

Guava Psidium guajava Somatic embryogenesis Zygotic embryo 

Notes

Acknowledgements

The financial support to author by various funding agencies like University Grant Commission, Council of Scientific and Industrial Research, Department of Biotechnology, Department of Science and Technology, Government of India is gratefully acknowledged. The author would like to extend his gratitude to the institutions viz. GITAM University, Visakhapatnam, India, Al-Ameen Arts Science and Commerce College, Bangalore, Jain University, Bangalore for their supports in continuing these researches.

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© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BiotechnologyGITAM Institute of Technology, GITAM UniversityVisakhapatnamIndia

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