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Affinity Membranes for Capture of Cells and Biological Substances

  • Rameshkumar Saranya
  • Rajendiran Murugan
  • Manasa Hegde
  • James Doyle
  • Ramesh Babu
Chapter

Abstract

Fundamental approaches of developing electrospun affinity membranes and their applications in cell capture and separation of biologically active substances are discussed. Basic principles of affinity membrane-based separation process and types of various surface modifications are described, to produce electrospun affinity nanofibers for filtration or fractionation of desired biological substances. The relevant advancements in electrospinning for novel surface modifications have been discussed with a special focus on coupled ligands and molecular imprinted polymers. Applications pertaining to cell capturing and filtration of biomolecules are reviewed in detail, with reference to recent reports on electrospun affinity nanofiber membranes for various antibody and protein purification.

Keywords

Affinity membranes Electrospinning Ligands Nanomaterials Nanofibers Immobilization Biomolecule Filtration Cell capture 

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Rameshkumar Saranya
    • 1
    • 2
  • Rajendiran Murugan
    • 3
    • 1
  • Manasa Hegde
    • 1
    • 2
  • James Doyle
    • 1
  • Ramesh Babu
    • 1
    • 2
    • 4
  1. 1.AMBER, CRANN Institute, Trinity College DublinDublinRepublic of Ireland
  2. 2.School of Physics, Trinity College DublinDublinRepublic of Ireland
  3. 3.OEM Diagnostics, Merck LtdCounty CorkRepublic of Ireland
  4. 4.BEACON - Bioeconomy Research CentreUniversity College DublinDublinRepublic of Ireland

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