Rheumatoid Factor (RF)

  • Rowa Yousef Alhabbab
Part of the Techniques in Life Science and Biomedicine for the Non-Expert book series (TLSBNE)


Rheumatoid arthritis is a chronic autoimmune disease associated with autoantibodies known as rheumatoid factor (RF). RF is produced by autoreactive B cells against self-IgG Fc regions. Therefore, RF is wieldy used in clinical laboratories as a biomarker for rheumatoid arthritis. This chapter describes the principle, applications, reagents, steps, results interpretation, and the limitations of the most commonly used test to detect RF.


Autoimmune RA RF ACPA Latex agglutination Gamma globulin 


  1. 1.
    Isaacs JD. The changing face of rheumatoid arthritis: sustained remission for all? Nat Rev Immunol. 2010;10(8):605–11.CrossRefPubMedGoogle Scholar
  2. 2.
    Burska AN, Hunt L, Boissinot M, Strollo R, Ryan BJ, Vital E, et al. Autoantibodies to posttranslational modifications in rheumatoid arthritis. Mediat Inflamm. 2014;2014:492873.Google Scholar
  3. 3.
    Nissim A, Winyard PG, Corrigall V, Fatah R, Perrett D, Panayi G, et al. Generation of neoantigenic epitopes after posttranslational modification of type II collagen by factors present within the inflamed joint. Arthritis Rheum. 2005;52(12):3829–38.CrossRefPubMedGoogle Scholar
  4. 4.
    Marcinkiewicz J, Biedron R, Maresz K, Kwasny-Krochin B, Bobek M, Kontny E, et al. Oxidative modification of type II collagen differentially affects its arthritogenic and tolerogenic capacity in experimental arthritis. Arch Immunol Ther Exp. 2004;52(4):284–91.Google Scholar
  5. 5.
    Anderton SM. Post-translational modifications of self antigens: implications for autoimmunity. Curr Opin Immunol. 2004;16(6):753–8.CrossRefPubMedGoogle Scholar
  6. 6.
    Shi J, Knevel R, Suwannalai P, van der Linden MP, Janssen GM, van Veelen PA, et al. Autoantibodies recognizing carbamylated proteins are present in sera of patients with rheumatoid arthritis and predict joint damage. Proc Natl Acad Sci U S A. 2011;108(42):17372–7.CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Eggleton P, Nissim A, Ryan BJ, Whiteman M, Winyard PG. Detection and isolation of human serum autoantibodies that recognize oxidatively modified autoantigens. Free Radic Biol Med. 2013;57:79–91.CrossRefPubMedGoogle Scholar
  8. 8.
    Abnova. Rheumatoid Factoer IgG ELISA kit. Available from: Accessed 6 Sept 2017.
  9. 9.
    Ingegnoli F, Castelli R, Gualtierotti R. Rheumatoid factors: clinical applications. Dis Markers. 2013;35(6):727–34.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Rowa Yousef Alhabbab
    • 1
  1. 1.Division of Applied Medical Sciences and Infectious Disease UnitKing Fahad Centre for Medical Research, King Abdulaziz UniversityJeddahSaudi Arabia

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