Rapid Plasma Reagin (RPR) Test

  • Rowa Yousef Alhabbab
Part of the Techniques in Life Science and Biomedicine for the Non-Expert book series (TLSBNE)


Syphilis is an example of a pathogen-caused infection that was once close to eradication, but is now reported to be infecting millions of patients worldwide. The disease ranges from asymptomatic to severe; therefore, patients suspected to be infected with syphilis should undergo syphilis testing, which is divided into non-treponemal (i.e., rapid plasma reagin) and treponemal tests. In this chapter, a full description of the non-treponemal (RPR) test principle, reagents, steps, and results interpretation are explored.


RPR Syphilis Treponema pallidum Non-treponemal test VDRL Agglutination 


  1. 1.
    Herring A, Ballard R, Mabey D, Peeling RW, Initiative WTSTDD. Evaluation of rapid diagnostic tests: syphilis. Nat Rev Microbiol. 2006;4(12 Suppl):S33–40.CrossRefPubMedGoogle Scholar
  2. 2.
    Peeling RW, Mabey DC. Syphilis. Nat Rev Microbiol. 2004;2(6):448–9.CrossRefPubMedGoogle Scholar
  3. 3.
    Lee JH, Lim CS, Lee MG, Kim HS. Comparison of an automated rapid plasma reagin (RPR) test with the conventional RPR card test in syphilis testing. BMJ Open. 2014;4(12):e005664.CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Larsen SA, Creighton ET. Rapid Plasma Reagin (RPR) 18-MM circle card test. CDC. 1996. Accessed 1 September 2017. Available from:
  5. 5.
    Angue Y, Yauieb A, Mola G, Duke T, Amoa AB. Syphilis serology testing: a comparative study of Abbot Determine, Rapid Plasma Reagin (RPR) card test and Venereal Disease Research Laboratory (VDRL) methods. P N G Med J. 2005;48(3-4):168–73.PubMedGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Rowa Yousef Alhabbab
    • 1
  1. 1.Division of Applied Medical Sciences and Infectious Disease UnitKing Fahad Centre for Medical Research, King Abdulaziz UniversityJeddahSaudi Arabia

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