Advertisement

Precipitation and Agglutination Reactions

  • Rowa Yousef Alhabbab
Chapter
Part of the Techniques in Life Science and Biomedicine for the Non-Expert book series (TLSBNE)

Abstract

The interaction of antibodies and antigens leads to the formation of immune complexes that are wieldy used in serology to detect antigens or antibodies of interest in a specific manner. There are several serological methods for detecting immune complexes; however, in this chapter, only precipitation, agglutination and hemagglutination are described.

Keywords

Precipitation Ring precipitation Gel diffusion Single diffusion Double diffusion Agglutination Direct agglutination Indirect agglutination Hemagglutination Direct hemagglutination Indirect hemagglutination 

References

  1. 1.
    Mak TW, Saunders ME. The immune response: basic and clinical principles. Amsterdam; Boston, MA: Elsevier/Academic; 2006. p. xx. 1194.Google Scholar
  2. 2.
    Coico R, Sunshine G. Immunology: a short course. 7th ed. Chichester, West Sussex; Hoboken, NJ: John Wiley & Sons Inc.; 2015.Google Scholar
  3. 3.
    Jacobsen C, Steensgaard J. Evidence of a two stage nature of precipitin reactions. Mol Immunol. 1979;16(8):571–6.CrossRefPubMedGoogle Scholar
  4. 4.
    Klimov A, Balish A, Veguilla V, Sun H, Schiffer J. Lu X, et al. Influenza virus titration, antigenic characterization, and serological methods for antibody detection. Methods Mol Biol. 2012;865:25–51.CrossRefPubMedGoogle Scholar
  5. 5.
    Mohan A, Saxena HM, Malhotra P. A comparison of titers of anti-Brucella antibodies of naturally infected and healthy vaccinated cattle by standard tube agglutination test, microtiter plate agglutination test, indirect hemagglutination assay, and indirect enzyme-linked immunosorbent assay. Vet World. 2016;9(7):717–22.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Singh DS, Das AK, Bhatia VN. Indirect haemagglutination test in the sero-diagnosis of amoebic and non-amoebic digestive disorders. J Assoc Physicians India. 1982;30(5):263–5.PubMedGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Rowa Yousef Alhabbab
    • 1
  1. 1.Division of Applied Medical Sciences and Infectious Disease UnitKing Fahad Centre for Medical Research, King Abdulaziz UniversityJeddahSaudi Arabia

Personalised recommendations