Immunofluorescence (IF) Assay

  • Rowa Yousef Alhabbab
Part of the Techniques in Life Science and Biomedicine for the Non-Expert book series (TLSBNE)


Immunofluorescence (IF) assay is largely used in clinical immunology laboratories, especially in autoimmune cases because it is a method that provides histological demonstration of antibodies and antigens related to the pathogenesis of the disease. This chapter covers the principles of the different IF test types, in addition to their reagents, steps, and results interpretation.


Immunofluorescence IF Autoimmune Direct IF Indirect IF Indirect complement fixation IF-CF Double immunofluorescence DIF 


  1. 1.
    Mohan KH, Pai S, Rao R, Sripathi H, Prabhu S. Techniques of immunofluorescence and their significance. Indian J Dermatol Venereol Leprol. 2008;74(4):415–9.CrossRefPubMedGoogle Scholar
  2. 2.
    Betterle C, Zanchetta R. The immunofluorescence techniques in the diagnosis of endocrine autoimmune diseases. Auto Immun Highlights. 2012;3(2):67–78.CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Huilgol SC, Bhogal BS, Black MM. Immunofluorescence of the immunobullous disorders: Part two: The clinical disorders. Indian J Dermatol Venereol Leprol. 1995;61(5):255–64.PubMedGoogle Scholar
  4. 4.
    Vassileva S. Immunofluorescence in dermatology. Int J Dermatol. 1993;32(3):153–61.CrossRefPubMedGoogle Scholar
  5. 5.
    Kern P, Kron M, Hiesche K. Measurement of antinuclear antibodies: assessment of different test systems. Clin Diagn Lab Immunol. 2000;7(1):72–8.CrossRefGoogle Scholar
  6. 6.
    Mengeloglu Z, Tas T, Kocoglu E, Aktas G, Karabork S. Determination of anti-nuclear antibody pattern distribution and clinical relationship. Pak J Med Sci. 2014;30(2):380–3.Google Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Rowa Yousef Alhabbab
    • 1
  1. 1.Division of Applied Medical Sciences and Infectious Disease UnitKing Fahad Centre for Medical Research, King Abdulaziz UniversityJeddahSaudi Arabia

Personalised recommendations