Immunofixation Electrophoresis (IFE)
Immunofixation electrophoresis (IFE) is a fundamental technique for detecting and quantifying plasma cell disorders such as monoclonal gammopathies. Samples with abnormal proteins such as M protein on the electrophoresis gel have to be confirmed and characterized by IFE. The principle, reagents, steps, and results interpretation of IFE are explained here.
KeywordsIFE M protein Monoclonal gammopathies MGUS Agarose gel Cellulose Acetate membrane
- 2.Kyle RA, Rajkumar SV. Management of monoclonal gammopathy of undetermined significance (MGUS) and smoldering multiple myeloma (SMM). Oncology (Williston Park). 2011;25(7):578–86.Google Scholar
- 4.Yılmaz M, Ozic C, Gok I. Principles of nucleic acid separation by agarose gel electrophoresis, gel electrophoresis – principles and basics. In Magdeldin S (Ed.), InTech. https://doi.org/10.5772/38654. 2012. Available from: https://www.intechopen.com/books/gel-electrophoresis-principles-and-basics/principles-of-nucleic-acid-separation-by-agarose-gel-electrophoresis. Accessed 30 September 2017.