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Enzyme Immunoassay (EIAs) and Enzyme-Linked Immunosorbent Assay (ELISA)

  • Rowa Yousef Alhabbab
Chapter
Part of the Techniques in Life Science and Biomedicine for the Non-Expert book series (TLSBNE)

Abstract

The enzyme-linked immunosorbent assay (ELISA) is a technique that can detect and quantify antigens or antibodies of interest in patient serum with high sensitivity and accuracy. ELISA is cost-effective; therefore, it is widely used in clinical and research laboratories. This chapter introduces the principles of the different types of ELISA, the reagents, and steps, and the most common method of interpreting the results.

Keywords

ELISA Direct ELISA Indirect ELISA Sandwich ELISA Competitive ELISA Substrate Enzyme 

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Rowa Yousef Alhabbab
    • 1
  1. 1.Division of Applied Medical Sciences and Infectious Disease UnitKing Fahad Centre for Medical Research, King Abdulaziz UniversityJeddahSaudi Arabia

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