Radioimmunoassay (RIA)

  • Rowa Yousef Alhabbab
Part of the Techniques in Life Science and Biomedicine for the Non-Expert book series (TLSBNE)


Radioimmunoassay (RIA) quantifies the amount of specific antigens in patient serum. This technique uses antigens labelled with radioisotopes, usually 125I, which requires specific safety measures; therefore, the use of RIA is very rare in clinical laboratories, especially with the presence of ELISA, which replaces the radioisotopes with enzyme. However, this chapter describes the principle, reagents, steps, and results interpretation of RIA.


RIA 125Radioisotopes Tracer NSB TB TC 


  1. 1.
    Annesley TM. It’s about the journey, not the destination: the birth of radioimmunoassay. 1960. Clin Chem. 2010;56(4):671–2.CrossRefPubMedGoogle Scholar
  2. 2.
    Grange RD, Thompson JP, Lambert DG. Radioimmunoassay, enzyme and non-enzyme-based immunoassays. Br J Anaesth. 2014;112(2):213–6.CrossRefPubMedGoogle Scholar
  3. 3.
    Gan SD, Patel KR. Enzyme immunoassay and enzyme-linked immunosorbent assay. J Invest Dermatol. 2013;133(9):e12.CrossRefPubMedGoogle Scholar
  4. 4.
    Institute of Isotopes Ltd. T4 [125I] RIA KIT. Izotop. Available from: Accessed 7 September 2017.
  5. 5.
    Phoenix Pharmaceuticals, Inc. General protocol for radio immunoassay kit. Phoenix. Available from: Accessed 10 September 2017.

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Rowa Yousef Alhabbab
    • 1
  1. 1.Division of Applied Medical Sciences and Infectious Disease UnitKing Fahad Centre for Medical Research, King Abdulaziz UniversityJeddahSaudi Arabia

Personalised recommendations