Radioimmunoassay (RIA) quantifies the amount of specific antigens in patient serum. This technique uses antigens labelled with radioisotopes, usually 125I, which requires specific safety measures; therefore, the use of RIA is very rare in clinical laboratories, especially with the presence of ELISA, which replaces the radioisotopes with enzyme. However, this chapter describes the principle, reagents, steps, and results interpretation of RIA.
KeywordsRIA 125I Radioisotopes Tracer NSB TB TC
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