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Production of Bio-oils from Microbial Biomasses

  • Laura Bardi
Chapter
Part of the Fungal Biology book series (FUNGBIO)

Abstract

Several yeasts and fungal strains are known for their ability to accumulate high amounts of lipids inside the cells. The interest for their utilisation at industrial level as sources of fats and oils (named SCO, single cell oils) was raised by various advantages presented by these microbial biomasses in comparison to other lipid sources, such as vegetable or animal feedstocks; among them, the possibility to obtain compounds with peculiar composition, the capability to use wastes and coproduct of other processes for cell growth and conversion into lipids, no dependence from seasonal and climatic trends and no requirement for agricultural lands. Biochemical pathways of lipid metabolism are well characterised in both oleaginous and non-oleaginous microorganisms, and their knowledge allows to orient cell physiology and metabolic engineering strategies aimed to improve quantity and quality of SCO production. Some SCO industrial processes have been developed, in particular in the fields of nutrition, of nutraceuticals and of biofuels.

Keywords

Single cell oils Oleaginous yeasts Fungi Lipids Fermentation Biofuels PUFA Nutraceuticals 

Notes

Acknowledgements

All the figures and tables were edited by Massimo Torta. The picture in Fig. 3.5 was taken by Fulvia Rosso.

References

  1. Ahamed A, Ahring BK (2011) Production of hydrocarbon compounds by endophytic fungi Gliocladium species grown on cellulose. Bioresour Technol 102:9718–9722CrossRefGoogle Scholar
  2. Anschau A, Xavier MCA, Hernalsteens S, Franco TT (2014) Effect of feeding strategies on lipid production by Lipomyces starkeyi. Bioresour Technol 157:214–222CrossRefPubMedGoogle Scholar
  3. Athenstaedt K, Jolive P, Boulard C, Zivy M, Negroni L, Nicaud JM, Chardot T (2006) Lipid particle composition of the yeast Yarrowia lipolytica depends on the carbon source. Proteomics 6:1450–1459CrossRefPubMedGoogle Scholar
  4. Bardi L, Crivelli C, Marzona M (1998) Esterase activity and release of ethyl esters of medium-chain fatty acids by Saccharomyces cerevisiae during anaerobic growth. Can J Microbiol 44:1171–1176CrossRefPubMedGoogle Scholar
  5. Bardi L, Cocito C, Marzona M (1999) Saccharomyces cerevisiae cell fatty acid composition and release during fermentation without aeration and in the absence of exogenous lipids. Int J Food Microbiol 47:133–140CrossRefPubMedGoogle Scholar
  6. Bellou S, Makria A, Sarris D, Michosa K, Rentoumia P, Celik A, Papanikolaou S, Aggelis G (2014) The olive mill wastewater as substrate for single cell oil production by Zygomycetes. J Biotechnol 170:50–59CrossRefPubMedGoogle Scholar
  7. Bellou S, Triantaphyllidou IE, Mizerakis P, Aggelis G (2016) High lipid accumulation in Yarrowia lipolytica cultivated under double limitation of nitrogen and magnesium. J Biotechnol 234:116–126CrossRefPubMedGoogle Scholar
  8. Belviso S, Bardi L, Biondi Bartolini A, Marzona M (2004) Lipid nutrition of Saccharomyces cerevisiae in winemaking. Can J Microbiol 50:657–667CrossRefGoogle Scholar
  9. Beopoulos A, Mrozova Z, Thevenieau F, Le Dall MT, Hapala I, Papanikolaou S, Chardot T, Nicaud JM (2008) Control of lipid accumulation in the yeast Yarrowia lipolytica. Appl Environ Microbiol 74(24):7779–7789CrossRefPubMedPubMedCentralGoogle Scholar
  10. Boulton CA, Ratledge C (1984) Cryptococcus terricolus, an oleaginous yeast re-appraised. Appl Microbiol Biotechnol 20(1):72–76CrossRefGoogle Scholar
  11. Brandenburg J, Blomqvist J, Pickova J, Bonturi N, Sandgren M, Passoth V (2016) Lipid production from hemicellulose with Lipomyces starkeyi in a pH regulated fed-batch cultivation. Yeast 2016, ISSY32 Special Issue,  https://doi.org/10.1002/yea
  12. Calvey CH, Su YK, Willis LB et al (2016) Nitrogen limitation, oxygen limitation, and lipid accumulation in Lipomyces starkeyi. Bioresour Technol 200:780–788CrossRefPubMedGoogle Scholar
  13. Certik M, Horenitzky R (1999) Supercritical CO2 extraction of fungal oil containing y-linolenic acid. Biotechnol Tech 13:11–15CrossRefGoogle Scholar
  14. Certik M, Shimizu S (1999) Biosynthesis and regulation of microbial polyunsaturated fatty acid production. J Biosci Bioeng 87(1):1–14CrossRefPubMedGoogle Scholar
  15. Certik M, Jana Megova J, Horenitzky R (1999) Effect of nitrogen sources on the activities of lipogenic enzymes in oleaginous fungus Cunninghamella echinulata. J Gen Appl Microbiol 45:289–293CrossRefPubMedGoogle Scholar
  16. Chatzifragkou A, Makri A, Belka A, Bellou S, Mavrou M, Mastoridou M, Mystrioti P, Onjaro G, Aggelis G, Papanikolaou S (2011) Biotechnological conversions of biodiesel derived waste glycerol by yeast and fungal species. Energy 36:1097–1108CrossRefGoogle Scholar
  17. Chuang LT, Chen DC, Nicaud JM, Madzak C, Chen YH, Huang YS (2010) Co-expression of heterologous desaturase genes in Yarrowia lipolytica. New Biotechnol 27:277–282CrossRefGoogle Scholar
  18. Daum G, Lee ND, Bard M, Dickson R (1998) Biochemistry, cell biology and molecular biology of lipids of Saccharomyces cerevisiae. Yeast 14:1471–1510CrossRefGoogle Scholar
  19. Dulermo T, Nicaud J-M (2011) Involvement of G3P shuttle and β-oxidation pathway into the control of TAG synthesis and lipid accumulation in Yarrowia lipolytica. Metab Eng 13:482–491CrossRefPubMedGoogle Scholar
  20. Easterling ER, French WT, Hernandez R, Licha M (2009) The effect of glycerol as a sole and secondary substrate on the growth and fatty acid composition of Rhodotorula glutinis. Bioresour Technol 100:356–361CrossRefPubMedGoogle Scholar
  21. Fakas S, Galiotou-Panayotou M, Papanikolaou S, Komaitis M, Aggelis G (2007) Compositional shifts in lipid fractions during lipid turnover in Cunninghamella echinulata. Enzym Microb Technol 40:1321–1327CrossRefGoogle Scholar
  22. Fakas S, Papanikolaou S, Batsos A, Galiotou-Panayotou M, Mallouchos A, Aggelis G (2009) Evaluating renewable carbon sources as substrates for single cell oil production by Cunninghamella echinulata and Mortierella isabellina. Biomass Bioenergy 33:573–580CrossRefGoogle Scholar
  23. Forfang K, Zimmermann B, Kosa G, Kohler A, Shapaval V (2017) FTIR Spectroscopy for evaluation and monitoring of lipid extraction efficiency for oleaginous fungi. PLOS ONE  https://doi.org/10.1371/journal.pone.0170611 January 24, 2017CrossRefPubMedPubMedCentralGoogle Scholar
  24. Henry SA (1982) Membrane lipids of yeast: biochemical and genetic study. In: Strathern JN, Jones EW, Broach JR (eds) Molecular biology of the yeast Saccharomyces cerevisiae: metabolism and gene expression. Cold Spring Harbor Laboratory, New York, pp 101–158Google Scholar
  25. Holdsworth JE, Veenhuis M, Ratledge C (1988) Enzyme activities in oleaginous yeasts accumulating and utilizing exogenous or endogenous lipids. J Gen Microbiol 134:2907–2915PubMedGoogle Scholar
  26. Hui L, Wan C, Hai-tao D, Xue-jiao C, Qi-fa Z, Yu-hua Z (2010) Direct microbial conversion of wheat straw into lipid by a cellulolytic fungus of Aspergillus oryzae A-4 in solid-state fermentation. Bioresour Technol 101:7556–7562CrossRefGoogle Scholar
  27. Jacklin A, Ratledge C, Wynn JP (2000) Lipid-to-gibberellin metabolic switching in Fusarium moniliforme via the action of sesamol. Biotechnol Lett 22(24):1983–1986CrossRefGoogle Scholar
  28. Kamisaka Y, Noda N, Sakai T, Kawasaki K (1999) Lipid bodies and lipid body formation in an oleaginous fungus, Mortierella ramanniana var. angulispora. Biochim Biophys Acta 1438(2):185–198CrossRefPubMedGoogle Scholar
  29. Koritala S, Hesseltine CW, Pryde EH, Mounts TL (1987) Biochemical modification of fats by microorganisms: a preliminary study. J Am Oil Chem Soc 64:509–513CrossRefGoogle Scholar
  30. Kuchler K, Daum G, Paltauf F (1986) Subcellular and submitochondrial localization of phospholipid-synthesizing enzymes in Saccharomyces cerevisiae. J Bacteriol 165(3):901–910CrossRefPubMedPubMedCentralGoogle Scholar
  31. Laoteng K, Mannontarat R, Tanticharoen M, Cheevadhanarak S (2000) Δ6-desaturase of Mucor rouxii with high similarity to plant Δ6-desaturase and its heterologous expression in Saccharomyces cerevisiae. Biochem Biophys Res Commun 279(1):17–22CrossRefPubMedGoogle Scholar
  32. Lee SK, Chou H, HamTS LTS, Keasling JD (2008) Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels. Curr Opin Biotechnol 19:556–563CrossRefPubMedGoogle Scholar
  33. Li YH, Liu B, Zhao ZB, Bai FW (2006) Optimized culture medium and fermentation conditions for lipid production by Rhodosporidium toruloides. Chin J Biotechnol 22(4):650–656CrossRefGoogle Scholar
  34. Meesters PA, Eggink G (1996) Isolation and characterization of a delta-9 fatty acid desaturase gene from the oleaginous yeast Cryptococcus curvatus CBS 570. Yeast 12:723–730CrossRefPubMedGoogle Scholar
  35. Meng X, Yang J, Xu X, Zhang L, Nie Q, Xian M (2009) Biodiesel production from oleaginous microorganisms. Renew Energy 34(1):1–5CrossRefGoogle Scholar
  36. Mlickova K, Luo Y, d’Andrea S, Pec P et al (2004a) Acyl-CoA oxidase, a key step for lipid accumulation in the yeast Yarrowia lipolytica. J Mol Catal B Enzym 28:81–85CrossRefGoogle Scholar
  37. Mlickova K, Roux E, Athenstaedt K, d’Andrea S, Daum G, Chardot T, Nicaud JM (2004b) Lipid accumulation, lipid body formation, and acyl-coenzyme A oxidases of the yeast Yarrowia lipolytica. Appl Environ Microbiol 70:3918–3924CrossRefPubMedPubMedCentralGoogle Scholar
  38. Moustogianni A, Bellou S, Triantaphyllidou IE, Aggelis G (2015) Feasibility of raw glycerol conversion into single cell oil by Zygomycetes under non-aseptic conditions. Biotechnol Bioeng 112(4):827–831CrossRefPubMedGoogle Scholar
  39. Nakajima T, Izu S (1993) Microbial production and purification of w-6 polyunsaturated fatty acids. In: Sinclair A, Gibson R (eds) Essential fatty acids and eicosanoids. AOCS, Champaign, pp 57–64Google Scholar
  40. Ozcan S, Johnston M (1999) Function and regulation of yeast hexose transporters. Microbiol Mol Biol Rev 63(3):554–569PubMedPubMedCentralGoogle Scholar
  41. Papanikolaou S, Aggelis G (2003) Selective uptake of fatty acids by the yeast Yarrowia lipolytica. Eur J Lipid Sci Technol 105:651–655CrossRefGoogle Scholar
  42. Papanikolaou S, Aggelis G (2011a) Lipids of oleaginous yeasts. Part I: biochemistry of single cell oil production. Eur J Lipid Sci Technol 113:1031–1051CrossRefGoogle Scholar
  43. Papanikolaou S, Aggelis G (2011b) Lipids of oleaginous yeasts. Part II: technology and potential applications. Eur J Lipid Sci Technol 113:1052–1073CrossRefGoogle Scholar
  44. Papanikolaou S, Chevalot I, Komaitis M, Marc I, Aggelis G (2002) Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures. Appl Microbiol Biotechnol 58:308–312CrossRefPubMedGoogle Scholar
  45. Papanikolaou S, Sarantou S, Komaitis M, Aggelis G (2004) Repression of reserve lipid turnover in Cunninghamella echinulata and Mortierella isabellina cultivated in multiple limited media. J Appl Microbiol 97:867–874CrossRefPubMedGoogle Scholar
  46. Passoth V (2014) Molecular mechanisms in yeast carbon metabolism: bioethanol and other biofuels. In: Piškur J, Compagno C (eds) Molecular mechanisms in yeast carbon metabolism. Springer-Verlag, BerlinGoogle Scholar
  47. Peralta-Yahya P, Keasling JD (2010) Advanced biofuel production in microbes. Biotechnol J 5:147–162CrossRefPubMedGoogle Scholar
  48. Ratledge C (1976) Microbial production of oils and fats. In: Birch GG, Parker KJ, Worgan JT (eds) Food from Waste. Applied Science Publishers, LondonGoogle Scholar
  49. Ratledge C (2013) Microbial oils: an introductory overview of current status and future prospects. OCL 20(6):D602CrossRefGoogle Scholar
  50. Ratledge C, Evans CT (1989) Lipids and their metabolism. In: Rose AH, Harrison JS (eds) The yeasts: metabolism and physiology of yeasts. Academic Press Limited, London, pp 369–434Google Scholar
  51. Ratledge C, Wynn JP (2002) The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms. Adv Appl Microbiol 51:1–51Google Scholar
  52. Rolph CE, Moreton RS, Small IS, Harwood JL (1986) Acyl lipid metabolism and fatty acid desaturation in the yeast Rhodotorula gracilis (CBS 3043). Biochem Soc Trans 14:712CrossRefGoogle Scholar
  53. Shanklin J, Whittle E, Fox BG (1994) Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase. Biochemistry 33(43):12787–12794CrossRefPubMedGoogle Scholar
  54. Shi S, Valle-Rodríguez JO, Siewers V, Nielsen J (2011) Prospects for microbial biodiesel production. Biotechnol J 6:277–285CrossRefPubMedGoogle Scholar
  55. Strobel GA (2014) Methods of discovery and techniques to study endophytic fungi producing fuel-related hydrocarbons. Nat Prod Rep 31:259CrossRefPubMedGoogle Scholar
  56. Strobel G, Knighton B, Kluck K, Ren Y, Livinghouse T, Griffin M, Spakowicz D, Sears J (2008) The production of myco-diesel hydrocarbons and their derivatives by the endophytic fungus Gliocladium roseum (NRRL 50072). Microbiologica 154:3319–3328Google Scholar
  57. Stukey JE, McDonough VM, Martin CE (1990) The OLE1 gene of Saccharomyces cerevisiae encodes the delta 9 fatty acid desaturase and can be functionally replaced by the rat stearoyl-CoA desaturase gene. J Biol Chem 265(33):20144–20149PubMedGoogle Scholar
  58. Tai M, Stephanopoulos G (2013) Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production. Metab Eng 15:1–9CrossRefPubMedGoogle Scholar
  59. Taylor FR, Parks LW (1978) Metabolic interconversion of free sterols and steryl esters in Saccharomyces cerevisiae. J Bacteriol 136(2):531–537PubMedPubMedCentralGoogle Scholar
  60. Tchakouteu SS, Chatzifragkou A, Kalantzi O, Apostolis A, Koutinas AA, Aggelis G, Papanikolaou S (2015a) Oleaginous yeast Cryptococcus curvatus exhibits interplay between biosynthesis of intracellular sugars and lipids. Eur J Lipid Sci Technol 117:657–672CrossRefGoogle Scholar
  61. Tchakouteu SS, Kalantzi O, Gardeli C, Koutinas AA, Aggelis G, Papanikolaou S (2015b) Lipid production by yeasts growing on biodiesel-derived crude glycerol: strain selection and impact of substrate concentration on the fermentation efficiency. J Appl Microbiol 118(4):911–927CrossRefPubMedGoogle Scholar
  62. Thevenieau F, Nicaud J-M (2013) Microorganisms as sources of oils. OCL 20(6):D603CrossRefGoogle Scholar
  63. Wolf J, Passarge J, Somsen OJ, Snoep JL, Heinrich R, Westerhoff HV (2000) Transduction of intracellular and intercellular dynamics in yeast glycolytic oscillations. Biophys J 78:1145–1153CrossRefPubMedPubMedCentralGoogle Scholar
  64. Wu S, Hu C, Jin C, Zhao X, Zhao ZB (2010) Phosphate limitation mediated lipid production by Rhodosporidium toruloides. Bioresour Technol 101:6124–6129CrossRefPubMedGoogle Scholar
  65. Wu S, Zhao X, Shen H, Wang Q, Zhao ZB (2011) Microbial lipid production by Rhodosporidium toruloides under sulfate-limited conditions. Bioresour Technol 102:1803–1807CrossRefPubMedGoogle Scholar
  66. Wynn JP, Ratledge C (2000) Evidence that the rate-limiting step for the biosynthesis of arachidonic acid in Mortierella alpina is at the level of the 18:3 to 20:3 elongase. Microbiology 146:2325–2331CrossRefPubMedGoogle Scholar
  67. Wynn JP, Hamidt AA, Ratledge C (1999) The role of malic enzyme in the regulation of lipid accumulation in filamentous fungi. Microbiology 145:1911–1917CrossRefPubMedGoogle Scholar
  68. Wynn JP, Ratledge C, Hamid AA, Li Y (2001) Biochemical events leading to the diversion of carbon into storage lipids in the oleaginous fungi Mucor circinelloides and Mortierella alpina. Microbiology 147(10):2857–2864CrossRefPubMedGoogle Scholar
  69. Yousuf A, Sannino F, Addorisio V, Pirozzi D (2010) Microbial conversion of olive oil mill wastewaters into lipids suitable for biodiesel production. J Agric Food Chem 58:8630–8635CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.CREA-IT, Council for Agricultural Research and Agricultural Economy – Research Centre for Engineering and Agro-Food ProcessingTurinItaly

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