Structure of Yeast Nuclear Pore Complexes
Nuclear pore complexes (NPCs) are large protein complex assemblies by about 30 different proteins, called nucleoporins (Nups), embedded in the nuclear envelope. Most of transport of molecules between cytoplasm and nucleus occurs through the NPCs. The research of yeast and vertebrate NPC structure made big progress in the past decades. This chapter first reviews recent advances of NPC structure and architecture by electron microscopy and super-resolution and then further overviews the progress of NPC structure and dynamic in living yeast cells by a single molecular detection approach called single-point edge-excitation sub-diffraction (SPEED) microscopy. In the last section, we will discuss the perspective about the structure of yeast NPCs.
This work is supported by National Natural Science Foundation of China (61575046, 11574056, and 31500599) and Science and Technology Commission of Shanghai Municipality (Shanghai Rising-Star Program, 16QA1400400).
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