Lantana aculeata L.-Mediated Zinc Oxide Nanoparticle-Induced DNA Damage in Sesamum indicum and Their Cytotoxic Activity Against SiHa Cell Line

  • Narendhran Sadasivam
  • Rajiv Periakaruppan
  • Rajeshwari Sivaraj


Zinc oxide nanoparticles were synthesized by biological method using aqueous extract of Lantana aculeata leaf and characterized by UV-visible spectroscopy, XRD, FTIR, FESEM, HRTEM and EDX analysis. The synthesized particles were highly stable and spherical, and particle size was in the range of 12–25 nm. The cytotoxicity activity of Lantana aculeata-mediated zinc oxide nanoparticles was evaluated by MTT assay against SiHa cervical cancer cell lines and confirmed that zinc oxide nanoparticles have cytotoxicity activity. The genotoxicity of ZnO nanoparticles was evaluated using comet assay and DNA laddering technique. ZnO nanoparticles in Sesamum indicum is yet to be confirmed in the comet assay and DNA laddering experiments. We detected increased level of DNA damage in concentration at 2000 mg L−1 treatment dose of ZnO nanoparticles in Sesamum indicum. The study thus confirms the toxicity potential of ZnO nanoparticles in both plant and human cancer cell line.


Comet assay Lantana aculeata Sesamum indicum SiHa cell line Zinc oxide nanoparticles 



I thank the management of Sri Krishna Arts and Science College for providing necessary facilities to carry out the research work and management of Karpagam Academy of Higher Education.


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© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Narendhran Sadasivam
    • 1
  • Rajiv Periakaruppan
    • 2
  • Rajeshwari Sivaraj
    • 3
  1. 1.Department of BiotechnologySri Krishna Arts and Science CollegeCoimbatoreIndia
  2. 2.Department of BiotechnologySchool of Life Sciences, Karpagam Academy of Higher EducationCoimbatoreIndia
  3. 3.Department of ChemistryGovernment Arts CollegeUdumalpetIndia

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