The basic principles for cryopreservation of sperm are not different from cryopreservation of embryos, eggs, and ovarian tissue. However freezing with sperm is much easier because there is such a minute amount of water in this small, otherwise highly specialized gamete. In fact very little advance has been made in sperm freezing research, because the results seen to be so adequate with the simplest of methods [1–22]. However I will describe, in addition to routine sperm cryopreservation, a simple new method for freezing individual sperm for difficult cases of TESE.
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