Cryopreservation of Sperm

  • Sherman Silber


The basic principles for cryopreservation of sperm are not different from cryopreservation of embryos, eggs, and ovarian tissue. However freezing with sperm is much easier because there is such a minute amount of water in this small, otherwise highly specialized gamete. In fact very little advance has been made in sperm freezing research, because the results seen to be so adequate with the simplest of methods [1–22]. However I will describe, in addition to routine sperm cryopreservation, a simple new method for freezing individual sperm for difficult cases of TESE.


  1. 1.
    Keel BA, Webster BW (1990) CRC handbook of the laboratory diagnosis and treatment of infertility. CRC Press, Boca Raton, FLGoogle Scholar
  2. 2.
    Mortimer D (1994) Practical laboratory andrology. Oxford University Press, New YorkGoogle Scholar
  3. 3.
    Morshedi M et al (1990) Cryopreserved/thawed semen for in vitro fertilization: results from fertile donors and infertile patients. Fertil Steril 54:1093–1099CrossRefPubMedGoogle Scholar
  4. 4.
    Oshima H, Burger HG (1993) Current topics in andrology. Japan Society of Andrology, TokyoGoogle Scholar
  5. 5.
    Hallak J et al (2000) Cryopreservation of human spermatozoa: comparison of TEST-yolk buffer and glycerol. Int J Fertil 45(1):38–42Google Scholar
  6. 6.
    Bhattacharya J et al (2006) A comparative study on TEST-yolk buffer and human sperm preservation medium on post thaw characteristics of human sperm from pre-freeze specimens. Fertil Steril 86:S200CrossRefGoogle Scholar
  7. 7.
    Hammadeh M et al (2001) Comparison between human sperm preservation medium and TEST-yolk buffer on protecting chromatin and morphology integrity of human spermatozoa in fertile and subfertile men after freeze-thawing procedure. J Androl 22(6):1012–1018CrossRefPubMedGoogle Scholar
  8. 8.
    Nallella KP et al (2004) Cryopreservation of human spermatozoa: comparison of two cryopreservation methods and three cryoprotectants. Fertil Steril 82:913–918CrossRefPubMedGoogle Scholar
  9. 9.
    Freezing Medium—TYB with Glycerol & Gentamicin (2016). Reproductive TechnologyGoogle Scholar
  10. 10.
    Sadri-Ardekani H, Atala A (2014) Testicular tissue cryopreservation and spermatogonial stem cell transplantation to restore fertility: from bench to bedside. Stem Cell Res Ther 5:68CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Sadri-Ardekani H, Akhondi MM, Vossough P, Maleki H, Sedighnejad S, Kamali K, Ghorbani B, van Wely M, van der Veen F, Repping S (2013) Parental attitudes toward fertility preservation in boys with cancer: context of different risk levels of infertility and success rates of fertility restoration. Fertil Steril 99:796–802CrossRefPubMedGoogle Scholar
  12. 12.
    Sadri-Ardekani H, Homburg CH, van Capel TM, van den Berg H, van der Veen F, van der Schoot CE, van Pelt AM, Repping S (2014) Eliminating acute lymphoblastic leukemia cells from human testicular cell cultures: a pilot study. Fertil Steril 101:1072–1078CrossRefPubMedGoogle Scholar
  13. 13.
    Sadri-Ardekani H, Mizrak SC, van Daalen SK, Korver CM, Roepers-Gajadien HL, Koruj M, Hoving S, de Reijk TM, de la Rossetta JJ, van der Veen F, de Rooij DG, Reeping S, van Pelt AM (2009) Propagation of human spermatogonial stem cells in vitro. JAMA 302:2127–2134CrossRefPubMedGoogle Scholar
  14. 14.
    Sadri-Ardekani H, Akhondi MA, van der Veen F, Repping S, van Pelt AM: In vitro propagation of human prepubertal spermatogonial stem cells. JAMA 2011, 305: 2416–2418.Google Scholar
  15. 15.
    Polge C (1957) Low-temperature storage of mammalian spermatozoa. Proc R Soc Lond B Biol Sci 147:498–508CrossRefPubMedGoogle Scholar
  16. 16.
    Bunge RG, Sherman JK (1953) Fertilizing capacity of frozen human spermatozoa. Nature 172:767–768CrossRefPubMedGoogle Scholar
  17. 17.
    Brinster RL (2007) Male germline stem cells: from mice to men. Science 316:404–405CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Brinster RL, Zimmermann JW (1994) Spermatogenesis following male germ-cell transplantation. Proc Natl Acad Sci U S A 91:11298–11302CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Bahadur G, Chatterjee R, Ralph D (2000) Testicular tissue cryopreservation in boys. Ethical and legal issues: case report. Hum Reprod 15:1416–1420CrossRefPubMedGoogle Scholar
  20. 20.
    Brinster RL, Avarbock MR (1994) Germline transmission of donor haplotype following spermatogonial transplantation. Proc Natl Acad Sci U S A 91:11303–11307CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Keros V, Hultenby K, Borgstrom B, Fridstrom M, Jahnukainen K, Hovatta O (2007) Methods of cryopreservation of testicular tissue with viable spermatogonia in pre-pubertal boys undergoing gonadotoxic cancer treatment. Hum Reprod 22:1384–1395CrossRefPubMedGoogle Scholar
  22. 22.
    Hovatta O (2001) Cryopreservation of testicular tissue in young cancer patients. Hum Reprod Update 7:378–383CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Sherman Silber
    • 1
    • 2
  1. 1.Infertility Center of St. LouisSt. Luke’s HospitalSt. LouisUSA
  2. 2.University of MichiganAnn ArborUSA

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