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Microtubule-Associated Protein 1 Light Chain 3 (LC3) Isoforms in RPE and Retina

  • Anuradha Dhingra
  • Desiree Alexander
  • Juan Reyes-Reveles
  • Rachel Sharp
  • Kathleen Boesze-Battaglia
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1074)

Abstract

Microtubule-associated protein 1 light chain 3 (MAP1LC3), a human homologue of yeast Atg8, is an essential component of autophagy. LC3 plays a critical role in hybrid degradation pathways in which some but not all components of autophagy are coupled with phagocytosis in a process known as LC3-associated phagocytosis (LAP). LC3 exists as three highly homologous isoforms in human (LC3A, LC3B, and LC3C) with two of these (LC3A and LC3B) in mouse. LC3B predominated in both fetal and adult human retinal pigment epithelium (RPE) relative to LC3A and LC3C, while in mouse RPE and neural retina, LC3A and LC3B were expressed at approximately equivalent levels. In situ hybridization studies localized LC3A and LC3B transcripts in the retina and RPE. LC3B protein was detected in C57Bl6/J RPE and retinal lysates and was absent in the LC3BKO mouse.

Keywords

LC3 isoforms Phagocytosis Retinal pigment epithelium 

Notes

Acknowledgments

Studies were supported by NIH grants EY010420 and EY026525 (KBB). The authors thank the PDM-live cell imaging core and the UPENN EM core for analyses.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Anuradha Dhingra
    • 1
  • Desiree Alexander
    • 1
  • Juan Reyes-Reveles
    • 1
  • Rachel Sharp
    • 1
  • Kathleen Boesze-Battaglia
    • 1
  1. 1.Department of BiochemistrySDM, University of PennsylvaniaPhiladelphiaUSA

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