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Pyrophosphate-Dependent Enzymes in Methanotrophs: New Findings and Views

  • Valentina N. Khmelenina
  • Olga N. Rozova
  • Ilya R. Akberdin
  • Marina G. Kalyuzhnaya
  • Yuri A. Trotsenko
Chapter

Abstract

Inorganic pyrophosphate (PPi) is a by-product of cellular anabolic processes whose recycle by inorganic pyrophosphatases is essential for biosynthetic reactions. While the metabolic role of PPi in anaerobic microorganisms is widely acknowledged, the contribution of PPi to central metabolic pathways in aerobic organisms is less understood. In methanotrophs, the enzyme 6-phosphofructokinase (PPi-PFK, EC 2.7.1.90) utilizes PPi as a phosphoryl donor in an energy-consuming stage of Embden-Meyerhof-Parnas glycolysis, i.e., phosphorylation of fructose-6-posphate into fructose-1,6-bisphosphate. Analysis of genomic data shows that the gene encoding PPi-PFK (pfp) is widespread among proteobacterial methanotrophs, while only rarely present in methylotrophs, thus implying a relationship between the PPi-metabolism and methane oxidation. Hence, a number of PPi-linked enzymes have been investigated, including membranous proton-translocating pyrophosphatase, pyruvate-phosphate dikinase, and phosphoenolpyruvate carboxykinase, in an attempt to uncover methanotrophic metabolic mechanisms that help compensate for the high energetic cost of methane oxidation and reset metabolic pathways for efficient C1-assimilation. The remarkable metabolic versatility of PPi reactions in methanotrophs allows them to grow under quite different environmental conditions, including aerobic respiration and semi-anaerobic formaldehyde fermentation.

Keywords

Inorganic pyrophosphate Pyrophosphatase, 6-phosphofructokinase Pyruvate, phosphate dikinase PPi-type phosphoenolpyruvate carboxykinase H+-translocating membranous pyrophosphatase Methanotrophs Glycolysis Gluconeogenesis 

Abbreviations

F6P

fructose-6-phosphate

FBP

fructose-1,6-bisphosphate

PFK

phosphofructokinase

PEP

phosphoenolpyruvate

PPDK

pyruvate, phosphate dikinase

PPi

inorganic pyrophosphate

PEPCK

phosphoenolpyruvate carboxykinase

PPases

pyrophosphatases

Ru5P

ribulose-5-phosphate

S7P

sedoheptulose-7-phosphate

H+-PPase

proton-translocating pyrophosphatase

Notes

Acknowledgments

The work was supported by the Russian Foundation for Basic Research #18-04-00771 and by the US Department of Energy Bioenergy Technologies Office (DOE-BETO under contract No. DE-AC36-08GO28308).

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Valentina N. Khmelenina
    • 1
  • Olga N. Rozova
    • 1
  • Ilya R. Akberdin
    • 2
  • Marina G. Kalyuzhnaya
    • 2
  • Yuri A. Trotsenko
    • 1
  1. 1.G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, RASPushchinoRussia
  2. 2.Department of Biology and Viral Information InstituteSan Diego State UniversitySan DiegoUSA

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