Culture of Vascular Cells In Vitro

  • Andreas Bikfalvi


Ross Harrison (1870–1959) was the first to attempt to culture tissues in vitro using culture dishes. In 1907 he had grown tadpole tissue in a coagulated interstitial liquid from frog using the hanging drop technique [104]. This technique is still used today to generate spheroids in vitro and to culture them.


  1. 104.
    Harrison R (1907) Observations on the living developing nerve fiber. Anat Rec, Proc Soc Exp Med, NY 1:116–128Google Scholar
  2. 105.
    Carrel A (1912) Pure cultures of cells. J Exp Med 16(2):165–168CrossRefPubMedPubMedCentralGoogle Scholar
  3. 106.
    Carrel A (1912) On the permanent life of tissues outside of the organism. J Exp Med 15(5):516–528CrossRefPubMedPubMedCentralGoogle Scholar
  4. 107.
    Eagle H (1955) Nutrition needs of mammalian cells in tissue culture. Science 122(3168):501–514CrossRefPubMedGoogle Scholar
  5. 108.
    Jaffe EA, Nachman RL, Becker CG, Minick CR (1973) Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 52(11):2745–2756. Scholar
  6. 109.
    Gimbrone MA Jr, Cotran RS, Folkman J (1973) Endothelial regeneration: studies with human endothelial cells in culture. Ser Haematol 6(4):453–455PubMedGoogle Scholar
  7. 110.
    Campbell GR, Uehara Y, Mark G, Burnstock G (1971) Fine structure of smooth muscle cells grown in tissue culture. J Cell Biol 49(1):21–34CrossRefPubMedPubMedCentralGoogle Scholar
  8. 111.
    Chamley-Campbell J, Campbell GR, Ross R (1979) The smooth muscle cell in culture. Physiol Rev 59(1):1–61CrossRefPubMedGoogle Scholar
  9. 112.
    Buzney SM, Frank RN, Robison WG Jr (1975) Retinal capillaries: proliferation of mural cells in vitro. Science 190(4218):985–986CrossRefPubMedGoogle Scholar
  10. 113.
    Weibel ER, Palade GE (1964) New cytoplasmic components in arterial endothelia. J Cell Biol 23:101–112CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2017

Authors and Affiliations

  • Andreas Bikfalvi
    • 1
  1. 1.Angiogenesis and Tumor Microenvironment LaboratoryUniversity of Bordeaux and National Institute of Health and Medical ResearchPessacFrance

Personalised recommendations