Conventional and Non-conventional Approach towards the Extraction of Bioorganic Phase

  • Sreenivasan Sasidharan
  • Shanmugapriya
  • Subramanion Lachumy Jothy
  • Soundararajan Vijayarathna
  • Nowroji Kavitha
  • Chern Ein Oon
  • Yeng Chen
  • Saravanan Dharmaraj
  • Ngit Shin Lai
  • Jagat R. Kanwar


Natural products such as natural food are the richest bio-resource of bioorganic compounds for modern medicines, nutraceuticals, food supplements and pharmaceutical applications. The research and application on natural food started with the extraction techniques that play an important role to the extraction quantity (Yield), quality (extracted phytochemicals) and also to the subsequent analyses accomplished to evaluate the biological and chemicals activities. Various types of technologies with different principles of extraction of bioorganic compounds are available today. Based on the literature the conventional extraction methods show better recoveries of bioorganic substances of natural food. Also, conventional extraction methods facilitate the extraction of high concentration of bioorganic substances with the safe solvents system such as pure ethanol. Moreover, conventional extraction methods is still widely used due to its simplicity. However, the conventional extraction methods is not always suitable for industrial uses due to long extraction time and large consumption of harmful solvents systems such as methanol. Therefore, modern non-conventional extraction methods could be an alternative extraction method. Hence, in spite of good results achieved with the conventional extraction methods, modern non-conventional extraction methods was established to search for a faster and better extraction method consuming less solvent, especially those that are unattractive in food industry. This chapter is intended to provide insights on conventional and non-conventional extraction methods with their advantages and disadvantage or limitation.


Extraction Natural products Solvent Quantity 


  1. Al-Marzouqi AH, Rao MV, Jobe B (2007) Comparative evaluation of SFE and steam distillation methods on the yield and composition of essential oil extracted from Spearmint (Mentha spicata). J Liq Chromatogr Relat Technol 30(4):463–475CrossRefGoogle Scholar
  2. Anderson S (2004) Soxtec: its principles and applications. In: Oil extraction and analysis-critical issue and comparative studies. AOCS Press, Champaign, pp 10–24Google Scholar
  3. Ansel HC, Popovich NG, Allen LV (1995) Pharmaceutical dosage forms and drug delivery systems. Lippincott Williams & Wilkins, PhiladelphiaGoogle Scholar
  4. Azwanida NN (2015a) A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants 4:1–6Google Scholar
  5. Azwanida NN (2015b) A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants 4:196Google Scholar
  6. Azwanida NN (2015c) A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants 4(196):2167–0412Google Scholar
  7. Bergonio KB, Perez MA (2016) The potential of male papaya (Carica papaya, L.) flower as a functional ingredient for herbal tea production. Indian J Tradit Knowl 15(1):41–49Google Scholar
  8. Chaisawadi S, Thongbute D, Methawiriyasilp W (2005) Preliminary study of antimicrobial activities on medicinal herbs of Thai food ingredients. In: Proceedings of the III WOCMAP congress on medicinal and aromatic plants: Bioprospecting and Ethnopharmacology, Chiang Mai, Thailand, 3–7 February 2003Google Scholar
  9. Chemat F, Rombaut N, Sicaire AG, Meullemiestre A, Fabiano-Tixier AS, Abert-Vian M (2016) Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrason Sonochem 34:540–560CrossRefGoogle Scholar
  10. Christensen LP (2008) Ginsenosides: Chemistry, biosynthesis, analysis, and potential health effects. In: Toldra F (ed) Advances in food and nutrition research, vol 82. Elsevier, Amsterdam, pp 1–99Google Scholar
  11. Delazar A, Nahar L, Hamedeyazdan S, Sarker SD (2012) Microwave-assisted extraction in natural oroducts. In: Sarker SD, Nahar L (eds) Natural products isolation, Methods in molecular biology, vol 864. Springer, New YorkGoogle Scholar
  12. Duraipandiyan V, Ayyanar M, Ignacimuthu S (2006) Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. BMC Complement Altern Med 6:35–41CrossRefGoogle Scholar
  13. Evans WC (2009) Trease and evans’ pharmacognosy, 16th edn. Saunders Ltd., LondonGoogle Scholar
  14. Fleisher A (1990) The poroplast extraction technique in the flavor and fragrance industry. Perfum Flav 15(5):27–36Google Scholar
  15. Fleisher A (1991) Water-soluble fractions of the essential oils. Perfum Flav 16(3):37–41Google Scholar
  16. Fowsiya J, Madhumitha G, Al-Dhabi NA, Arasu MV (2016) Photocatalytic degradation of congo red using Carissa edulis extract capped zinc oxide nanoparticles. J Photochem Photobiol B 162:395–401CrossRefGoogle Scholar
  17. Ganzler K, Salgo A (1986) Microwave extraction: a novel sample preparation method for chromatography. J Chromatogr A 371:299–306CrossRefGoogle Scholar
  18. Ganzler K, Salgo A (1987) Microwave-extraction-a new method superseding traditional soxhlet extraction. Z-LebensmUnters Forsch 184:274–276CrossRefGoogle Scholar
  19. Ganzler K, Szinai I, Salgo A (1990) Effective sample preparation method for extracting biologically active compounds from different matrixes by a microwave technique. J Chromatogr A 520:257–262CrossRefGoogle Scholar
  20. Hamburger M, Baumann D, Adler S (2004) Supercritical carbon dioxide extraction of selected medicinal plants–effects of high pressure and added ethanol on yield of extracted substances. Phytochem Anal 15:46–54CrossRefGoogle Scholar
  21. Handa SS, Khanuja SPS, Longo G, Rakesh DD (2008) Extraction technologies for medicinal and aromatic Plants, 1st ed, no. 66. United Nations Industrial Development Organization and the International Centre for Science and High Technology. Trieste, ItalyGoogle Scholar
  22. He B, Zhang LL, Yue XY, Liang J, Jiang J, Gao XL, Yue PX (2016) Optimization of ultrasound-assisted extraction of phenolic compounds and anthocyanins from blueberry (Vaccinium ashei) wine pomace. Food Chem 204:70–76CrossRefGoogle Scholar
  23. Hitchcock RT (2004) Radio frequency and microwave radiation, 3rd edn. American Industrial Hygiene Association, VirginiaCrossRefGoogle Scholar
  24. Jennings WG, Wohleb RH, Wohlers NW (1981) High pressure soxhlet extractor. In: McHugh M, Krukonis V (eds) Supercritical fluid extraction, 2nd edn. Butterworth-Heinemann, USAGoogle Scholar
  25. Jones WP, Kinghorn AD (2005) Extraction of plant secondary metabolites. In: Sarker SD, Latif Z, Gray AI (eds) Natural products isolation, Methods of biotechnology series, 2nd edn. Humana Press, Totowa, pp 323–350Google Scholar
  26. Kaufmann B, Christen P (2002) Recent extraction techniques for natural products: microwave-assisted extraction and pressurized solvent extraction. Phytochem Anal 13:105–113CrossRefGoogle Scholar
  27. Khajeh M, Yamini Y, Sefidkon F, Bahrafimar N (2004) Comparison of essential oil composition of Carum copticum obtained by supercritical carbon dioxide extraction and hydrodistillation methods. J Food Chem 86:587–591CrossRefGoogle Scholar
  28. Kwon JH, Bélanger JMR, Pare JRJ (2003) Optimization of microwave-assisted extraction (MAP) for ginseng components by response surface methodology. J Agric Food Chem 51(7):1807–1810CrossRefGoogle Scholar
  29. Li Y, Fabiano-Tixier AS, Tomao V, Cravotto G, Chemat F (2012) Green ultrasound-assisted extraction of carotenoids based on the bio-refinery concept using sunflower oil as an alternative solvent. Ultrason Sonochem 20:12–18CrossRefGoogle Scholar
  30. Luque de Castro MD, García-Ayuso LE (1998) Soxhlet extraction of solid materials: an outdated technique with a promising innovative future. Anal Chim Acta 369:1–2):1–10CrossRefGoogle Scholar
  31. Luque de Castro MD, Priego-Capote F (2010) Soxhlet extraction: past and present panacea. J. Chromatogr A 1217(16):2383–2389CrossRefGoogle Scholar
  32. Madhumitha G, Rajakumar G, Roopan SM, Rahuman AA, Priya KM, Saral AM, Khan FRN, Khanna VG, Velayutham K, Jayaseelan C, Kamaraj C, Elango G (2012) Acaricidal, insecticidal and larvicidal efficacy of fruit peel aquoues extract of Annona squamosa and its compounds against blood feeding parasites. Parasitol Res 111:2189–2199CrossRefGoogle Scholar
  33. Mandal SC, Mandal V, Das AK (2015) Classification of extraction methods. In: Essentials of botanical extraction: principles and applications. Academic Press, Cambridge, MA, pp 83–136CrossRefGoogle Scholar
  34. Nahar L, Sarker SD (2012) Supercritical fluid extraction in natural products analyses. In: Sarker SD, Nahar L (eds) Natural products isolation, Methods in molecular biology, vol 864. Springer, New York, NYGoogle Scholar
  35. Naji G, Mellouk H, Rezzouget SA, Allaf K (2008) Extraction of essential oils of juniper berries by instantaneous controlled pressure-drop: improvement of DIC process and comparison with the steam distillation. J Essent Oil Bear Plants 11(4):356–364CrossRefGoogle Scholar
  36. Nwankwo N, Anthony EA, Chime J, Belonwu E (2013) Effects of separation on the phytochemical properties and antimicrobial activity of extracts and a fraction of the African Mistletoe (Loranthusmicranthus Linn) leaves. J Nat Sci Res 3:44–51Google Scholar
  37. Petigny L, Perino-Issartier S, Wajsman J, Chemat F (2013) Batch and continuous ultrasound assisted extraction of Boldo Leaves (Peumus boldus Mol.) Int J Mol Sci 14:5750–5764CrossRefGoogle Scholar
  38. Pradal D, Vauchel P, Decossin S, Dhulster P, Dimitrov K (2016) Kinetics of ultrasound-assisted extraction of antioxidant polyphenols from food by-products: extraction and energy consumption optimization. Ultrason Sonochem 32:137–146CrossRefGoogle Scholar
  39. Rohloff J (1999) Monoterpene composition of essential oil from peppermint (mentha piperita) with regard to leaf position using solid-phase microextraction and gas chromatography/mass spectrometry analysis. J Agric Food Chem 47:3782CrossRefGoogle Scholar
  40. Sadjia B, Naima S, Chahrazed B (2012) Extraction of thyme (Thymus pallecens de Noé) essential oil by steam-distillation, steam-diffusion and hydro-distillation processes: optimization of operating conditions and antioxidant activity. J Essent Oil Bear Plants 15(2):336–347CrossRefGoogle Scholar
  41. Saleh IA, Vinatoru M, Mason TJ, Abdel-Azim NS, Aboutabl EA, Hammouda FM (2016) A possible general mechanism for ultrasound-assisted extraction (UAE) suggested from the results of UAE of chlorogenic acid from Cynara scolymus L. (artichoke) leaves. Ultrason Sonochem 31:330–336CrossRefGoogle Scholar
  42. Sasidharan S, Chen Y, Saravanan D, Sundram K, Yoga Latha L (2011) Extraction, isolation and characterization of bioactive compounds from plants’ extracts. Afr J Tradit Complement Altern Med 8:1–10PubMedGoogle Scholar
  43. Seidel V (2012) Initial and bulk extraction of natural products isolation. Methods Mol Biol 864:27–41CrossRefGoogle Scholar
  44. Singh J (2008) Maceration, percolation and infusion techniques for the extraction of medicinal and aromatic plants. Extraction technologies for medicinal and aromatic plants, vol 67, pp 32–35Google Scholar
  45. Soxhlet F (1879) Die gewichtsanalytische Bestimmung des Milchfettes. Dinglers Polytechnisches J 232:461–465Google Scholar
  46. Sun Y, Liu D, Chen J, Ye X, Yu D (2011) Effects of different factors of ultrasound treatment on the extraction yield of the all-trans-beta-carotene from citrus peels. Ultrason Sonochem 18:243–249CrossRefGoogle Scholar
  47. Tariq S, Imran M, Mushtaq Z, Asghar N (2016) Phytopreventive antihypercholesterolmic and antilipidemic perspectives of zedoary (Curcuma Zedoaria Roscoe.) herbal tea. Lipids Health Dis 15(1):39CrossRefGoogle Scholar
  48. Teng H, Lee WY (2014) Antibacterial and antioxidant activities and chemical compositions of volatile oils extracted from Schisandra chinensis Baill. seeds using simultaneous distillation extraction method, and comparison with Soxhlet and microwave-assisted extraction. Biosci Biotechnol Biochem 78(1):79–85CrossRefGoogle Scholar
  49. Thuery J (1992) Microwaves and matter. In: Grant EH (ed) Microwaves: Industrial, Scientific and Medical Applications, Part 1. Artech House, London, pp 88–125Google Scholar
  50. Toma M, Vinatoru M, Paniwnyk L, Mason TJ (2001) Investigation of the effects of ultrasound on vegetal tissues during solvent extraction. Ultrason Sonochem 8:137–142CrossRefGoogle Scholar
  51. Trusheva B, Trunkova D, Bankova V (2007a) Different extraction methods of biologically active components from propolis: a preliminary study. Chem Cent J 1(1):13CrossRefGoogle Scholar
  52. Trusheva B, Trunkova D, Bankova V (2007b) Different extraction methods of biologically active components from propolis: a preliminary study. Chem Cent J 1:13CrossRefGoogle Scholar
  53. Xu C, Chou GX, Wang ZT (2010) A new diterpene from the leaves of Andrographis paniculata Nees. Fitoterapia 81(6):610–613CrossRefGoogle Scholar
  54. Zhang QA, Shen Y, Fan XH, Martin JF, Wang X, Song Y (2015) Free radical generation induced by ultrasound in red wine and model wine: an EPR spin-trapping study. Ultrason Sonochem 27:96–101CrossRefGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Sreenivasan Sasidharan
    • 1
  • Shanmugapriya
    • 1
  • Subramanion Lachumy Jothy
    • 1
  • Soundararajan Vijayarathna
    • 1
  • Nowroji Kavitha
    • 1
  • Chern Ein Oon
    • 1
  • Yeng Chen
    • 2
  • Saravanan Dharmaraj
    • 3
  • Ngit Shin Lai
    • 1
  • Jagat R. Kanwar
    • 4
  1. 1.Institute for Research in Molecular Medicine (INFORMM)Universiti Sains Malaysia11800 USMMalaysia
  2. 2.Dental Research & Training Unit, and Oral Cancer Research and Coordinating Centre (OCRCC), Faculty of DentistryUniversity of Malaya50603 Kuala LumpurMalaysia
  3. 3.Faculty of MedicineUniversiti Sultan Zainal Abidin, Medical CampusKuala TerengganuMalaysia
  4. 4.Nanomedicine-Laboratory of Immunology and Molecular Biomedical Research (LIMBR), School of Medicine (SoM), Faculty of HealthDeakin UniversityGeelongAustralia

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