Microdissection for Study of Cochlear Anatomy

  • Charles G. Wright
  • Peter S. Roland


Temporal bone microdissection offers a versatile laboratory method for the study of inner ear anatomy and pathology. It is well suited for use in parallel with electron microscopy and conventional histologic techniques. The microdissection method provides a three-dimensional perspective that is especially useful in research relating to cochlear implantation.


Temporal bone Cochlea Anatomy Microdissection Electron microscopy Histology 


  1. 1.
    Hawkins JE Jr, Johnsson L-G. Microdissection and surface preparations of the inner ear. In: Smith CA, Vernon JA, editors. Handbook of auditory and vestibular research methods. Springfield, IL: Charles C Thomas; 1976. p. 5–52.Google Scholar
  2. 2.
    Wright CG, Roland PS. Temporal bone microdissection for anatomic study of cochlear implant electrodes. Coch Implant Int. 2005;6:159–68.CrossRefGoogle Scholar
  3. 3.
    Nomura Y, Schuknecht HF. The efferent fibers in the cochlea. Ann Otol Rhinol Laryngol. 1965;74:289–302.CrossRefPubMedGoogle Scholar
  4. 4.
    Nomura Y, Kirikae J. Innervation of the human cochlea. Ann Otol Rinol Laryngol. 1967;76:57–68.CrossRefGoogle Scholar
  5. 5.
    Ishii T, Murakami Y, Balogh K Jr. Acetylcholinesterase activity in the efferent nerve fibers of the human inner ear. Ann Otol Rhinol Laryngol. 1967;76:69–82.CrossRefPubMedGoogle Scholar
  6. 6.
    Liberman MC, Rosowski JJ, Lewis RF. Physiology and pathophysiology. In: Merchant SN, Nadol Jr JB, editors. Schuknecht’s pathology of the ear. 3rd ed. Shelton, CT: People’s Medical Publishing House-USA Ltd; 2010. p. 97–134.Google Scholar
  7. 7.
    Johnsson L-G, Hawkins JE Jr. Sensory and neural degeneration with aging, as seen in microdissections of the human inner ear. Ann Otol Rhinol Larngol. 1972;81:179–93.CrossRefGoogle Scholar
  8. 8.
    Wright CG, Meyerhoff WL. Microdissection in the study of human temporal bone morphology. Ann Otol Rhinol Laryngol. 1989;98(Suppl 143):25–8.CrossRefGoogle Scholar
  9. 9.
    Ulualp SO, Wright CG, Pawlowski KS, Roland PS. Histopathological basis of hearing impairment in Wolf-Hirschhorn syndrome. Laryngoscope. 2004;114:1426–30.CrossRefPubMedGoogle Scholar
  10. 10.
    Moore BC. Dead regions in the cochlea: conceptual foundations, diagnosis, and clinical applications. Ear Hear. 2004;25:98–116.CrossRefPubMedGoogle Scholar
  11. 11.
    Zhang T, Dorman MF, Gifford R, Moore BC. Cochlear dead regions constrain the benefit of combining acoustic stimulation with electric stimulation. Ear Hear. 2014;35:410–7.CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Gantz BJ, Turner C, Gfeller KE, Lowder MW. Preservation of hearing in cochlear implant surgery: advantages of combined electrical and acoustical speech processing. Laryngoscope. 2005;115:796–802.CrossRefPubMedGoogle Scholar
  13. 13.
    Roland PS, Wright CG. Surgical aspects of cochlear implantation: mechanisms of insertional trauma. In: Moller AR, editor. Cochlear and brainstem implants, Adv Otorhinolaryngol, vol. 64. Basel: Karger; 2006. p. 11–30.Google Scholar
  14. 14.
    Shepherd RK, Colreavy MP. Surface microstructure of the perilymphatic space: implications for cochlear implants and cell- or drug-based therapies. Arch Otolaryngol Head Neck Surg. 2004;130:518–23.CrossRefPubMedGoogle Scholar
  15. 15.
    Rask-Andersen H, Schrott-Fischer A, Pfaller K, Glueckert R. Perilymph/modiolar communication routes in the human cochlea. Ear Hear. 2006;27:457–65.CrossRefPubMedGoogle Scholar

Suggested Reading

  1. Adams JC, Liberman MC. Anatomy. In: Merchant SN, Nadol Jr JB, editors. Schuknecht’s pathology of the ear. 3rd ed. Shelton, CT: People’s Medical Publishing House-USA Ltd; 2010. p. 53–95.Google Scholar
  2. Anson BJ, Donaldson JA. Surgical anatomy of the temporal bone and ear. 2nd ed. Philadelphia: WB Saunders; 1973.Google Scholar
  3. Avci E, Nauwelaers T, Lenarz T, Hamacher V, Kral A. Variations in microanatomy of the human cochlea. J Comp Neurol. 2014;522:3245–61.CrossRefPubMedPubMedCentralGoogle Scholar
  4. Bredberg G. Cellular pattern and nerve supply of the human organ of Corti. Acta Otolaryngol. 1968;Suppl. 236:1–135.Google Scholar
  5. Friedmann I, Ballantyne J. Ultrastructural atlas of the inner ear. London: Butterworth; 1984.Google Scholar
  6. Gulya AJ, Gulya A. Schuknecht’s anatomy of the temporal bone with surgical implications. 3rd ed. Boca Raton, FL: CRC Press; 2007.CrossRefGoogle Scholar
  7. Johnsson L-G, Felix H, Gleeson M, Pollak A. Observations on the pattern of sensorineural degeneration in the human cochlea. Acta Otolaryngol. 1990;Suppl. 470:88–96.Google Scholar
  8. Raphael Y, Altschuler RA. Structure and innervation of the cochlea. Brain Res Bull. 2003;60:397–422.CrossRefGoogle Scholar
  9. Rask-Andersen H, Liu W, Erixon E, Kinnefors A, Pfaller K, Schrott-Fischer A, et al. Human cochlea: anatomical characteristics and their relevance for cochlear implantation. Anat Rec. 2012;295:1791–811.CrossRefGoogle Scholar
  10. Wysocki J. Dimensions of the human vestibular and tympanic scalae. Hear Res. 1999;135:39–46.CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Charles G. Wright
    • 1
  • Peter S. Roland
    • 1
  1. 1.Department of Otolaryngology-Head and Neck SurgeryUniversity of Texas Southwestern Medical CenterDallasUSA

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