Quantitative Live Cell FLIM Imaging in Three Dimensions

  • Alix Le Marois
  • Klaus SuhlingEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1035)


In this chapter, the concept of fluorescence lifetime and its utility in quantitative live cell imaging will be introduced, along with methods to record and analyze FLIM data. Relevant applications in 3D tissue and live cell imaging, including multiplexed FLIM detection, will also be detailed.


FLIM techniques Time-correlated single photon counting (TCSPC) Multi-channel FLIM Multi-modal FLIM Optical sectioning FLIM data analysis 


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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Department of PhysicsKing’s College LondonLondonUK

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