Sensitive Crystallization Patterns in Oral Cancer
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Pfeiffer, a German scientist in 1938, first developed the crystallization test, which baffled many researchers ever since. Later on, cupric chloride crystallization test was widely used in the literature to investigate its efficacy in detection of various malignancies. Studies on oral cancer also proved its effectiveness for early detection. The crystallization appearance called “transverse form” is regarded as a hallmark pattern in malignancies. It is postulated that increased concentration of polyamines and diamines in blood of cancer patients as well as altered protein structure is responsible for formation of this peculiar, signature pattern. However, in normal healthy patients, the cupric chloride crystallization pattern is characterized by an eccentrically placed center of gravity and radiating crystals without any disturbances. In this chapter, we have reviewed the crystallization test with emphasis on potential mechanisms, crystallization test procedure and methodology, image interpretation, crystal patterns, and all studies conducted on oral cancer. The qualities of reliability, simplicity, cost-effective, and noninvasive nature of crystallization test make it an efficient tool for sensitive detection of oral cancer.
KeywordsCrystallization test Cupric chloride Oral cancer Early detection Malignancy
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