The Addition Effect of Fermented Aegle marmelos Fruit and Bamboo Shoots in Cattle Feces Slurry to the Reduction of Ammonia Gas Emission
Abstract
This study aimed to determine the effect of fermented Aegle marmelos and bamboo shoots in reducing ammonia gas emission from cattle feces slurry. Aegle marmelos, bamboo shoots, molasses, and water were fermented for 10 days in anaerobic condition. The fermentation product was then investigated for chemical properties, sensory aspects, microbial content as well as the growth on the solid and liquid medium by measuring the optical density (OD600nm), and the concentration of ammonia gas emission. The study was performed by addition at different level 0% (control), 1%, 3%, and 5% of fermentation product and 1% of commercial starter EM-4 into cattle feces slurry to observe the ability of ammonia gas emission reduction. The data of fermentation product such as physical and chemical properties, sensory, and the growth of microbes from fermentation product on the solid and liquid medium, the optical density (OD600nm), and microbial growth after the treatment were descriptively analyzed. Ammonia gas concentration was analyzed with factorial completely randomized design patterns (5 × 4) when it showed significant differences followed by Duncan’s new multiple range test (DMRT). The results of the study showed that the fermentation product has pH 3.05, temperature 26 °C, color brown to black, and fresh scent and is slightly sour and fragrant. Microbial fermentation observation results showed that it could be grown on a solid and liquid medium. The addition of fermentation product has significantly decreased (p < 0.01) the ammonia gas emission.
Keywords
Aegle marmelos fruit Ammonia emission Bamboo shoots Cattle feces FermentationNotes
Acknowledgments
We thank the Laboratory of Animal Skin, By-product, and Waste Technology, Faculty of Animal Science, Gadjah Mada University, for conducting and facilitating in all research stages.
References
- 1.Direktorat_Jenderal_Peternakan: Statistik Peternakan. http://ditjennak.deptan.go.id/index.php?page=statistikpeternakan&action=info (2016). Accessed 22 April 2016
- 2.Zhu, J.: A review of microbiology in swine manure odor control. Agric. Ecosyst. Environ. 78, 93–106 (2000)Google Scholar
- 3.Mackie, R.I., et al.: Biochemical identification and biological origin of key odor components in livestock waste. J. Anim. Sci. 76, 1331–1342 (1998)CrossRefPubMedGoogle Scholar
- 4.Nakai, Y.: Animal waste management and microorganisms. Anim. Sci. J. 72, 1–3 (2001)CrossRefGoogle Scholar
- 5.McCubbin, D.R., et al.: Livestock ammonia management and particulate-related health benefits. Environ. Sci. Technol. 36, 1141–1146 (2002)CrossRefPubMedGoogle Scholar
- 6.Sasaki, H., et al.: A survey of ammonia-assimilating micro-organisms in cattle manure composting. J. Appl. Microbiol. 99, 1356–1363 (2005)CrossRefPubMedGoogle Scholar
- 7.Yamamoto, N., et al.: Diversity and abundance of ammonia-oxidizing bacteria and ammonia-oxidizing archaea during cattle manure composting. Microb. Ecol. 60, 807–815 (2010)CrossRefPubMedGoogle Scholar
- 8.Noble, R., et al.: Olfactory response to mushroom composting emissions as a function of chemical concentration. J. Environ. Qual. 30, 760–767 (2001)CrossRefPubMedGoogle Scholar
- 9.McCrory, D.F., Hobbs, P.J.: Additives to reduce ammonia and odor emissions from livestock wastes: a review. J. Environ. Qual. 30, 345–355 (2001)CrossRefPubMedGoogle Scholar
- 10.Beline, F., et al.: Nitrogen transformations during anaerobically stored 15 N-labelled pig slurry. Bioresour. Technol. 64, 83–88 (1998)Google Scholar
- 11.Ndegwa, P.M., et al.: A review of ammonia emission mitigation techniques for concentrated animal feeding operations. Biosyst. Eng. 100, 453–469 (2008)CrossRefGoogle Scholar
- 12.Hartung, J., Phillips, V.: Control of gaseous emissions from livestock buildings and manure stores. J. Agric. Eng. Res. 57, 173–189 (1994)CrossRefGoogle Scholar
- 13.Maity, P., et al.: Biological activities of crude extracts and chemical constituents of Bael, Aegle marmelos (L.) Corr. Indian J. Exp. Biol., 47. 849–861 (2009)Google Scholar