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Specificity and Cross-order Activity of Bacillus thuringiensis Pesticidal Proteins

  • Kees van Frankenhuyzen
Chapter

Abstract

Published data on insecticidal activity of crystal proteins from B. thuringiensis were incorporated into a database on Bt toxin specificity. At the end of 2013, 158 of the 329 known holotypes were tested against 252 species distributed across 95 families in 25 orders, 8 classes, and 5 phyla. Thirty of the 158 proteins were reported to have no pesticidal activity, 59 were active against Lepidoptera, 42 against Diptera, 40 against Coleoptera, 10 against Hemiptera, 4 against Hymenoptera, and 1 against Orthoptera. Reports of toxicity to Trichoptera, Neuroptera, and Siphonaptera were not substantiated. Twelve proteins were reported to have activity against non-Arthropod species in the phyla Platyhelminthes and Nematoda. Activity outside orders of primary specificity was reported for 28 proteins affecting 75 taxa and was substantiated by reasonable evidence (mortality estimates) for 21 proteins and 51 taxa. Substantiated cross-activity occurred in 14 primary rank families across three classes of pesticidal proteins (Cry, Cyt, and Vip). Within the phylum Arthropoda, cross-activities were substantiated for 12 proteins (Cry1Ca, Cry1Ia, Cry4Aa, Cry8Da, Cry10Aa, Cry11Aa, Cry30Fa, Cry30Ga, Cry51Aa, Cry54Aa, Cry56Aa, Vip1A/Vip2A) affecting species across two orders, five proteins (Cry1Ac, Cry1Ba, Cry3Aa, Cry2Aa, Cyt1Aa) affecting three orders, and one protein (Cyt1Ba) affecting four orders, all within the class Insecta. Testing of insecticidal proteins against species in other Arthropod classes has not produced conclusive evidence of lethal activity outside the class Insecta. Cross-phylum activity was substantiated only for three insecticidal proteins (Cry1Ab, Cry2Ab, Cry3Bb), which affected nematode growth and reproduction at high dose levels. Target toxicity of B. thuringiensis pesticidal proteins can be broadly classified as being high when proteins are active in the 0.01–0.10 μg/ml range (e.g., Diptera-active proteins), medium when active in the 0.10–10 μg/ml range (Lepidoptera-, some Diptera-, and most Coleoptera-active proteins), and low when active in the 10–1000 μg/ml range (Coleoptera- and Nematode-active proteins). These categories are based on 25%, (0.1 μg/ml), 50% (1 μg/ml), and 75% (10 μg/ml) percentiles of nonparametric distribution of 50% lethal concentration estimates (LC50s, n = 256) when pooled across all tested proteins and taxa. Toxicity (LC50s) outside the order of primary specificity was quantified for 16 proteins and 24 taxa. Compared to toxicity ranges established for Diptera-, Coleoptera-, Lepidoptera-, and Nematoda-active proteins, 15 cross-activities were in the low- (10–1000 μg/ml), seven in the medium- (0.10–10), and two in the high-toxicity range (0.01–0.10 μg/ml). Activities outside a protein’s suite of orders that is normally affected were mostly in the low-toxicity range. This was the case for toxicity of Cry1Ab, Cry1Ac, Cry2Aa, Cry3Aa, Cry4Aa, Cry11Aa, and Cyt1Aa to Hemiptera (aphids), of Cry51Aa to Hemiptera (Lygus spp.), and of Cyt1Ba to Hymenoptera (sawflies). The exception is high toxicity of Cry3Aa to fire ants (Hymenoptera). Activities that are within the suite of orders normally affected but outside a protein’s primary-order affinity were often in the medium-toxicity range of corresponding reference proteins. This group includes dipteran toxicity of Cry1Ac, lepidopteran toxicity of Cry8Da, and coleopteran toxicity of Cry1Ba, Cry1Ia, Cry10Aa, Cyt1Aa, and Cyt1Ba. Dipteran toxicity of Cry1Ba, Cry1Ca, and Cyt1Ba occurs at high dose levels compared to Diptera-active reference proteins. Cross-activities of B. thuringiensis pesticidal proteins need to be viewed with caution until they are confirmed through independent testing. Nevertheless, current evidence suggests that cross-activities are not uncommon, having been substantiated for ~13% of the 158 proteins tested to date, and may be more prevalent considering that one-third of proteins that were tested against species in two or more orders were confirmed to be cross-active. One-third of reported cross-activities fall within the range of toxicities exhibited by order-specific proteins that are commonly used in pest control applications. Cross-activities therefore should be considered when designing or approving the use of B. thuringiensis pesticidal proteins in pest control applications.

Keywords

Bacillus thuringiensis Artropod Pesticidal-proteins Toxicity Cross-activities Pest-control 

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© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Great Lakes Forestry Centre, Canadian Forest ServiceSault Ste. MarieCanada

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