P Deficiency: A Major Limiting Factor for Rhizobial Symbiosis

  • Alvaro Sanz-Saez
  • Fermín Morales
  • Cesar Arrese-Igor
  • Iker Aranjuelo
Chapter

Abstract

Together with nitrogen (N), phosphorus (P) has been described as the main plant macronutrient limiting growth. Although P is abundant in many soils, its availability for plants is low. For this reason, P is provided to plants largely through the application of P-enriched fertilizers. However, since rock phosphate reserves (the main source of P) are predicted to be depleted in the near future, it is crucial to understand the processes linked with a better P use efficiency. P is a target structural constituent of energetic compounds (ATP, ADP), nucleic acids, phosphate sugars, etc., that are essential for cell metabolism and plant development. Current knowledge highlights that low P availability negatively affects above- and below-ground organ growth, as a consequence, in part, of poor photosynthetic performance. While essential for all plants, the P requirement of N2-fixing plants has been described as larger than that of non N2-fixing plants, mainly as a consequence of the large P demand for biological N2 fixation (BNF) processes. Moreover, three main factors have been suggested to affect BNF under low P conditions: carbon supply, N-feedback and O2 diffusion have been identified as the main factors conditioning N2 fixation under low P availability conditions. In this chapter, we summarize current knowledge regarding P content in plant performance, with special emphasis on N2-fixing plants and the symbiotic relationship.

Keywords

Biological N2 fixation Growth Legumes Nodule P deficiency Rhizobium Symbiosis 

Notes

Acknowledgments

This work was funded by the Spanish National Research and Development Programme (AGL2014-56561-P), the “I-COOP Suelos y Legumbres” Programme (2016SU0016) and their corresponding FEDER funding, and Aragón Government (A03 research group).

References

  1. Ae N, Arihara J, Okada K, Yoshihara T, Johansen C (1990) Phosphorus uptake by pigeon pea and its role in cropping systems of the Indian subcontinent. Science 248:477–480CrossRefPubMedGoogle Scholar
  2. Agbariah K-T, Roth-Bejerano N (1990) The effect of blue light on energy levels in epidermal strips. Physiol Planta 78:100–104CrossRefGoogle Scholar
  3. Alexandratos N, Bruinsma J (2012) World agriculture towards 2030/2050: the 2012 revision. ESA Working paper No. 12–03. FAO, RomeGoogle Scholar
  4. Almeida JPF, Hartwig UA, Frehner M, Nosberger J, Luscher A (2000) Evidence that P deficiency induces N feedback regulation of symbiotic N2 fixation in white clover (Trifolium repens L.) J Exp Bot 51:1289–1297PubMedGoogle Scholar
  5. Ankomah AB, Zapata F, Hardarson G, Danso SKA (1996) Yield, nodulation, and N2 fixation by cowpea cultivars at different phosphorus levels. Biol Fert Soils 22:10–15CrossRefGoogle Scholar
  6. Anuradha M, Narayanan A (1991) Promotion of root elongation by phosphorus deficiency. Plant Soil 136:273–275CrossRefGoogle Scholar
  7. Aranjuelo I, Arrese-Igor C, Molero G (2014) Nodule performance within a changing environmental context. J Plant Physiol 98:32–39Google Scholar
  8. Bieleski RL (1973) Phosphate pools, phosphate transport, and phosphate availability. Annu Rev Plant Physiol 24:225–252CrossRefGoogle Scholar
  9. Bottrill DE, Possingham JV, Kriedemann PE (1970) The effect of nutrient deficiencies on photosynthesis and respiration in spinach. Plant Soil 32:424–438CrossRefGoogle Scholar
  10. Brooks A (1986) Effects of phosphorus nutrition on ribulose-1,5-bisphosphate carboxylase activation, photosynthetic quantum yield and amounts of some Calvin cycle metabolites in spinach leaves. Aust J Plant Physiol 13:221–237CrossRefGoogle Scholar
  11. Bumb BL, Baanante CA (1996) The role of fertilizer in sustaining food security and protecting the environment. Food, Agriculture, and the Environment, Discussion Paper 17. International Food Policy Research Institute, Washington DCGoogle Scholar
  12. Cabeza RA et al (2014) RNA-seq transcriptome profiling reveals that Medicago truncatula nodules acclimate N2 fixation before emerging P deficiency reaches the nodules. J Exp Bot 65(20):6035–6048CrossRefPubMedPubMedCentralGoogle Scholar
  13. Cooper J, Lombardi R, Boardman D, Carliell-Marquet C (2011) The future distribution and production of global phosphate rock reserves. Resour Conserv Recycl 57:78–86CrossRefGoogle Scholar
  14. Cordell D (2010) The story of phosphorus. Sustainability implications of global phosphorus scarcity for food security. Linköping University, LinköpingGoogle Scholar
  15. De Haes HAU, Jansen J, Van Der Weijden LA, Smit WJAL (2009) Phosphate – from surplus to shortage. In: Policy memorandum of the Steering Committee for Technology Assessment. Ministry of Agriculture, Nature and Food Quality, UtrechtGoogle Scholar
  16. Divito GA, Sadras VO (2014) How do phosphorus, potassium and sulphur affect plant growth and biological nitrogen fixation in crop and pasture legumes? A meta-analysis. Field Crop Res 156:161–171CrossRefGoogle Scholar
  17. Dyson T (1999) World food trends and prospects to 2025. Proc Natl Acad Sci USA 96:5929–5936CrossRefPubMedPubMedCentralGoogle Scholar
  18. Edixhoven JD, Gupta J, Savenije HHG (2014) Recent revisions of phosphate rock reserves and resources: a critique. Earth Syst Dynam 5:491–507. doi:10.5194/esd-5-491-2014 CrossRefGoogle Scholar
  19. Fredeen AL, Rao IM, Terry N (1989) Influence of phosphorus nutrition on growth and carbon partitioning in Glycine max. Plant Physiol 89:225–230CrossRefPubMedPubMedCentralGoogle Scholar
  20. Gálvez L, González EM, Arrese-Igor C (2005) Evidence for carbon flux shortage and strong carbon/nitrogen interactions in pea nodules at early stages of water stress. J Exp Bot 56: 2551–2561Google Scholar
  21. Gilroy S, Jones DL (2000) Through form to function Broot hair development and nutrient uptake. Trends Plant Sci 3:56–60CrossRefGoogle Scholar
  22. González EM, Gálvez L, Royuela M, Aparicio-Tejo PM, Arrese-Igor C (2001) Insights into the regulation of nitrogen fixation in pea nodules: lessons from drought, abscisic acid and increased photoassimilate availability. Agronomie 21:607–613Google Scholar
  23. Gordon AJ, Mitchell DF, Ryle GJA, Powell CE (1987) Diurnal production and utilization of photosynthates in nodulated white clover. J Exp Bot 38:84–98Google Scholar
  24. Gunawardena SFBN, Danso SKA, Zapata F (1992) Phosphorus requirements and nitrogen accumulation by 3 mungbean (Vigna radiata (L) Welzek) cultivars. Plant Soil 147:267–274Google Scholar
  25. Halsted M, Lynch J (1996) Phosphorus responses of C3 and C4 species. J Exp Bot 47:497–505CrossRefGoogle Scholar
  26. Harrison MJ (1997) The arbuscular mycorrhizal symbiosis: an underground association. Trends Plant Sci 2:54–60CrossRefGoogle Scholar
  27. Hernández G, Valdés-López O, Ramírez M, Goffard N, Weiller G, Aparicio-Fabre R, Fuentes SI, Erban A, Kopka J, Udvardi MK, Vance CP (2009) Global changes in the transcript and metabolic profiles during symbiotic nitrogen fixation in phosphorus-stressed common bean plants. Plant Physiol 151:1221–1238CrossRefPubMedPubMedCentralGoogle Scholar
  28. Herold A (1980) Regulation of photosynthesis by sink activity—the missing link. New Phytol 86:131–144CrossRefGoogle Scholar
  29. Holford ICR (1997) Soil phosphorus: its measurement, and its uptake by plants. Aust J Soil Res 35:227–239CrossRefGoogle Scholar
  30. Horst WJ (1995) The role of the apoplast in aluminium toxicity and resistance of higher plants: a review. Z Pflanzenernähr Bodenkd 158:419–428CrossRefGoogle Scholar
  31. Horst WJ, Wang Y, Eticha D (2010) The role of the root apoplast in aluminium-induced inhibition of root elongation and in aluminium resistance of plants: a review. Ann Bot 106:185–197CrossRefPubMedPubMedCentralGoogle Scholar
  32. Hunt S, Layzell DB (1993) Gas exchange of legume nodules and the regulation of nitrogenase activity. Annu Rev Plant Physiol Plant Mol Biol 44:483–511CrossRefGoogle Scholar
  33. Irigoyen JJ, Goicoechea N, Antolín MC, Pascual I, Sánchez-Díaz M, Aguirreolea J, Morales F (2014) Growth, photosynthetic acclimation and yield quality in legumes grown under climate change simulations: an updated survey. Plant Sci 226:22–29CrossRefPubMedGoogle Scholar
  34. Jakobsen I (1985) The role of phosphorus in nitrogen-fixation by young pea-plants (Pisum-Sativum). Physiol Plant 64:190–196CrossRefGoogle Scholar
  35. Jasinski SM (2011) Phosphate rock, mineral commodity summaries. U.S. Geological Survey, RestonGoogle Scholar
  36. Jebara M, Aouani ME, Payre H, Drevon JJ (2005) Nodule conductance varied among common bean (Phaseolus vulgaris) genotypes under phosphorus deficiency. J Plant Physiol 162:309–315Google Scholar
  37. Juszczuk IM, Rychter AM (2002) Pyruvate accumulation during phosphate deficiency stress of bean roots. Plant Physiol Biochem 40(9):783–788CrossRefGoogle Scholar
  38. Khamis S, Chaillou S, Lamaze T (1990) CO2 assimilation and partitioning of carbon in maize plants deprived of orthophosphate. J Exp Bot 41:1619–1625CrossRefGoogle Scholar
  39. Kleinert A, Venter M, Kossmann J, Valentine A (2014) The reallocation of carbon in P deficient lupins affects biological nitrogen fixation. J Plant Physiol 171:1619–1624CrossRefPubMedGoogle Scholar
  40. Kouas S, Labidi N, Debez A, Abdelly C (2005) Effect of P on nodule formation and N fixation in bean. Agron Sustain Dev 25:389–393CrossRefGoogle Scholar
  41. Krapp A, Stitt M (1995) An evaluation of direct and indirect mechanisms for the sink regulation of photosynthesis in spinach—changes in gas exchange, carbohydrates, metabolites, enzyme activities and steady-state transcript levels after cold-girdling source leaves. Planta 195:313–323CrossRefGoogle Scholar
  42. Lauer MJ, Pallardy SG, Blevins DG, Randall DD (1989) Whole leaf carbon exchange characteristics of phosphate deficient soybeans (Glycine max L.) Plant Physiol 91:848–854CrossRefPubMedPubMedCentralGoogle Scholar
  43. Le Roux MR, Ward CL, Botha FC, Valentine AJ (2006) Routes of pyruvate synthesis in phosphorus-deficient lupin roots and nodules. New Phytol 169(2):399–408CrossRefPubMedGoogle Scholar
  44. Le Roux MR, Khan S, Valentine AJ (2008) Organic acid accumulation may inhibit N2 fixation in phosphorus-stressed lupin nodules. New Phytol 177(4):956–964CrossRefPubMedGoogle Scholar
  45. Lea PJ, Forde BG (1994) The use of mutants and transgenic plants to study amino acid metabolism plant. Cell Environ 17:541–556Google Scholar
  46. Liu Y, Villalba G, Ayres RU, Schroder H (2008) Global phosphorus flows and environmental impacts from a consumption perspective. J Ind Ecol 12:229–247CrossRefGoogle Scholar
  47. Lodwig E, Poole P (2003) Metabolism of rhizobium bacteroids. Crit Rev Plant Sci 22:37–78CrossRefGoogle Scholar
  48. Lynch J, Lauch HA, Epstein E (1991) Vegetative growth of the common bean in response to phosphorus nutrition. Crop Sci 31:380–387CrossRefGoogle Scholar
  49. Marschner H (1995) Mineral nutrition of higher plants. Academic, LondonGoogle Scholar
  50. Marschner H, Dell B (1994) Nutrient uptake in mycorrhizal symbiosis. Plant Soil 159:89–102CrossRefGoogle Scholar
  51. Miao SJ, Qiao YF, Han XZ, An M (2007) Nodule formation and development in soybeans (Glycine max L.) in response to phosphorus supply in solution culture. Pedosphere 17:36–43CrossRefGoogle Scholar
  52. Mousavishalmani MA, Sagheb N, Hobbi MS, Rafh H, Khorasani A (2002) Fertilizer P distribution into different parts of plant and soil under trickle fertigation on tomato by 32P. In: 17th Word Congress Soil Science, Paper 2286, ThailandGoogle Scholar
  53. Nasr Esfahani MN, Kusanob M, Nguyend KH, Watanabee Y, Ha CV, Saitoc K, Sulieman S, Herrera-Estrella L, Tran LSP (2016) Adaptation of the symbiotic Mesorhizobium–chickpea relationship to phosphate deficiency relies on reprogramming of whole-plant metabolism. Proc Natl Acad Sci USA 113(32):E4610–E4619CrossRefPubMedPubMedCentralGoogle Scholar
  54. Peoples MB, Herridge DF, Ladha JK (1995) Biological nitrogen fixation: an efficient source of nitrogen for sustainable agricultural production. Plant Soil 174:3–28CrossRefGoogle Scholar
  55. Pieters AJ, Paul MJ, Lawlor DW (2001) Low sink demand limits photosynthesis under Pi deficiency. J Exp Bot 52:1083–1091CrossRefPubMedGoogle Scholar
  56. Plaxton WC (2004) Plant response to stress: biochemical adaptations to phosphate deficiency. In: Goodman R (ed) Encyclopedia of plant and crop science. Marcel Dekker, New York, pp 976–980CrossRefGoogle Scholar
  57. Plesnicar M, Kastori R, Petrovic N, Pankovic D (1994) Photosynthesis and chlorophyll fluorescence in sunflower (Helianthus annuus L.) leaves as affected by phosphorus nutrition. J Exp Bot 45:919–924CrossRefGoogle Scholar
  58. Qiu J, Israel DW (1992) Diurnal starch accumulation and utilization in phosphorus-deficient soybean plants. Plant Physiol 98:316–323CrossRefPubMedPubMedCentralGoogle Scholar
  59. Radin JW (1990) Responses of transpiration and hydraulic conductance to root temperature in nitrogen- and phosphorus-deficient cotton seedlings. Plant Physiol 92:855–857CrossRefPubMedPubMedCentralGoogle Scholar
  60. Ragothama KG (1999) Phosphate acquisition. Annu Rev Plant Physiol Plant Mol Biol 50:665–693CrossRefGoogle Scholar
  61. Rao IM, Terry N (1989a) Leaf phosphate status, photosynthesis and carbon partitioning in sugar beet. I. Changes in growth, gas exchange and Calvin cycle enzymes. Plant Physiol 90:814–819CrossRefPubMedPubMedCentralGoogle Scholar
  62. Rao IM, Terry N (1989b) Leaf phosphate status, photosynthesis and carbon partitioning in sugar beet. II. Diurnal changes in sugar phosphates, adenylates and nicotinamide nucleotides. Plant Physiol 90:820–826CrossRefPubMedPubMedCentralGoogle Scholar
  63. Rao IM, Terry N (1990) Leaf phosphate status, photosynthesis and carbon partitioning in sugar beet. III. Diurnal changes in carbon partitioning and carbon export. Plant Physiol 92:29–36CrossRefPubMedPubMedCentralGoogle Scholar
  64. Rao IM, Terry N (1995) Leaf phosphate status, photosynthesis, and carbon partitioning in sugar beet. IV: changes with time following increased supply of phosphate to low phosphate plants. Plant Physiol 107:1313–1321CrossRefPubMedPubMedCentralGoogle Scholar
  65. Ribet J, Drevon JJ (1995) Increase in conductance to oxygen and in oxygen uptake of soybean nodules under limiting phosphorus nutrition. Physiol Plant 94:298–304CrossRefGoogle Scholar
  66. Ribot C, Wang Y, Poirier Y (2008) Expression analysis of three members of the AtPHO1 family reveal differential interactions between signaling pathways involved in phosphate deficiency and the responses to auxin, cytokinin, and abscisic acid. Planta 227:1025–1036CrossRefPubMedGoogle Scholar
  67. Rogers A, Gibon Y, Stitt M, Morgan PB, Bernacchi CJ, Ort DR, Long SP (2006) Increased C availability at elevated carbon dioxide concentration improves N assimilation in a legume. Plant Cell Environ 29:1651–1658CrossRefPubMedGoogle Scholar
  68. Sawada S, Usuda H, Tsukui T (1992) Participation of inorganic orthophosphate in regulation of the ribulose-1,5-bisphosphate carboxylase activity in response to changes in the photosynthetic source-sink balance. Plant Cell Physiol 33:943–949Google Scholar
  69. Schachtman DP, Reid RJ, Ayling SM (1998) Phosphorus uptake by plants: from soil to cell. Plant Physiol 116:447–453Google Scholar
  70. Scholz RW, Wellmer FW (2016) Comment on: “recent revisions of phosphate rock reserves and resources: a critique” by Edixhoven et al. (2014)—clarifying comments and thoughts on key conceptions, conclusions and interpretation to allow for sustainable action. Earth Syst Dynam 7:103–117CrossRefGoogle Scholar
  71. Schröder JJ, Cordell D, Smit AL, Rosemarin A (2010) Sustainable use of phosphorus, EU tender EN V. B.1/ETU/2009/0025. Plant Research International, Business Unit Agrosystems, Wageningen UR, WageningenGoogle Scholar
  72. Schulze J, Drevon JJ (2005) P-deficiency increases the O2 uptake per N2 reduced in alfalfa. J Exp Bot 56:1779–1784CrossRefPubMedGoogle Scholar
  73. Schulze J, Adgo E, Merbach W (1999) Carbon costs associated with N2 fixation in Vicia faba L. and Pisum sativum L. over a 14-day period. Plant Biol 1:625–631CrossRefGoogle Scholar
  74. Schulze J, Tesfaye M, Litjens R, Bucciarelli B, Trepp G, Miller S et al (2002) Malate plays a central role in plant nutrition. Plant Soil 247:133–139CrossRefGoogle Scholar
  75. Schulze J, Mohamed MAN, Carlsson G, Drevon JJ (2011) Phosphorus deficiency decreases nitrogenase activity but increases proton efflux in N2-fixing Medicago truncatula. Plant Physiol Biochem 49:458–460CrossRefPubMedGoogle Scholar
  76. Serraj R, Sinclair TR (1996) Inhibition of nitrogenase activity and nodule oxygen permeability by water deficit. J Exp Bot 47:1067–1073CrossRefGoogle Scholar
  77. Smit AL, Bindraban PS, Schröder JJ, Conijn JG, Van Der Meer HG (2009) Phosphorus in agriculture: global resources trends and developments. Plant Research International B.V, WageningenGoogle Scholar
  78. Smith FW, Jackson WA, van den Berg PJ (1990) Internal phosphorus flows during development of phosphorus stress in Stylosanthes hamata. Aust J Plant Physiol 17:451–464CrossRefGoogle Scholar
  79. Sulieman S, Ha CV, Schulze J, Tran L-SP (2013) Growth and nodulation of symbiotic Medicago truncatula at different levels of phosphorus availability. J Exp Bot 64(10):2701–2712CrossRefPubMedPubMedCentralGoogle Scholar
  80. Sulieman S, Schulze J, Tran LSP (2014) N-feedback regulation is synchronized with nodule carbon alteration in Medicago truncatula under excessive nitrate or low phosphorus conditions. J Plant Physiol 171:407–410CrossRefPubMedGoogle Scholar
  81. Terry N, Ulrich A (1973) Effects of phosphorus deficiency on the photosynthesis and respiration of leaves of sugar beet. Plant Physiol 51:43–47CrossRefPubMedPubMedCentralGoogle Scholar
  82. Treeby MT, Van Steveninck RFM, de Vries HM (1987) Quantitative estimates of phosphorus concentrations within Lupinus luteus leaflets by means of electron probe X-ray microanalysis. Plant Physiol 85:331–334CrossRefPubMedPubMedCentralGoogle Scholar
  83. Turnbull TL, Warren CR, Adams MA (2007) Novel mannose-sequestration technique reveals variation in subcellular orthophosphate pools do not explain the effects of phosphorus nutrition on photosynthesis in Eucalyptus globulus seedlings. New Phytol 176:849–861CrossRefPubMedGoogle Scholar
  84. United Nations, Department of Economic and Social Affairs, Population Division (2015). World population prospects: The 2015 revision, key findings and advance tables, Working paper No. ESA/P/WP.241Google Scholar
  85. USGS (2011) Mineral commodity summaries, Phosphate Rock. US Geological Survey, Washington, DCGoogle Scholar
  86. USGS (2014) Mineral commodity survey: mineral commodity summaries. US Geological Survey, Washington, DCGoogle Scholar
  87. Vaccari DA (2009) Phosphorus, a looming crisis. Sci Am 300:42–47CrossRefGoogle Scholar
  88. Vadez V, Rodier F, Payre H, Drevon JJ (1996) Nodule permeability to O2 and nitrogenase-linked respiration in bean genotypes varying in the tolerance of N2 fixation to P deficiency. Plant Physiol Biochem 34(6):871–878Google Scholar
  89. Vance CP (2001) Symbiotic nitrogen fixation and phosphorus acquisition. Plant nutrition in a world of declining renewable resources. Plant Physiol 127:390–397CrossRefPubMedPubMedCentralGoogle Scholar
  90. Vance CP, Graham PH, Allan DL (2000) Biological nitrogen fixation: phosphorus Ba critical future need? In: Pederosa FO, Hungria M, Yates MG, Newton WE (eds) Nitrogen fixation: from molecules to crop productivity. Kluwer, Dordrecht, pp 506–514Google Scholar
  91. Vardien W, Mesjasz-Przybylowicz J, Przybylowicz WJ, Wang YD, Steenkamp ET, Valentine AJ (2014) Nodules from Fynbos legume Virgilia divaricata have high functional plasticity under variable P supply levels. J Plant Physiol 171:1732–1739CrossRefPubMedGoogle Scholar
  92. Vardien W, Steenkampb ET, Valentine AJ (2016) Legume nodules from nutrient-poor soils exhibit high plasticity of cellular phosphorus recycling and conservation during variable phosphorus supply. J Plant Physiol 191:73–81Google Scholar
  93. von Uexküll HR, Mutert E (1995) Global extent, development and economic impact of acid soils. Plant Soil 171:1–15CrossRefGoogle Scholar
  94. Vysotskaya LB, Trekozova AW, Kudoyarova GR (2016) Effect of phosphorus starvation on hormone content and growth of barley plants. Acta Physiol Plant 38:108CrossRefGoogle Scholar
  95. Walker DA, Sivak MN (1985) Can phosphate limit photosynthetic carbon assimilation in vivo? Physiol Veg 23:829–841Google Scholar
  96. Walker DA, Sivak MN (1986) Photosynthesis and phosphate: a cellular affair? Trends Biochem Sci 11:176–179CrossRefGoogle Scholar
  97. Warren CR (2011) How does P affect photosynthesis and metabolite profiles of Eucalyptus globulus? Tree Physiol 31:727–739CrossRefPubMedGoogle Scholar
  98. Warren CR, Adams MA (2002) Phosphorus affects growth and partitioning of nitrogen to Rubisco in Pinus pinaster. Tree Physiol 22:11–19CrossRefPubMedGoogle Scholar
  99. Xu HX, Weng XY, Yang Y (2007) Effect of phosphorus deficiency on the photosynthetic characteristics of rice plants. Russ J Plant Physiol 54:741–748CrossRefGoogle Scholar
  100. Yang N, Zavisic A, Pena R, Polle A (2016) Phenology, photosynthesis, and phosphorus in European beech (Fagus sylvatica L.) in two forest soils with contrasting P contents. J Plant Nutr Soil Sci 179:151–158CrossRefGoogle Scholar
  101. Zheng SJ (2010) Crop production on acidic soils: overcoming aluminium toxicity and phosphorus deficiency. Ann Bot 106:183–184CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Alvaro Sanz-Saez
    • 1
  • Fermín Morales
    • 2
  • Cesar Arrese-Igor
    • 3
  • Iker Aranjuelo
    • 4
  1. 1.Division of Plant SciencesUniversity of MissouriColumbiaUSA
  2. 2.Estación Experimental de Aula Dei (EEAD), CSIC, Dpto. Nutrición VegetalZaragozaSpain
  3. 3.Dpto. Ciencias del Medio NaturalUniversidad Pública de NavarraPamplonaSpain
  4. 4.Instituto de Agrobiotecnología (IdAB)Universidad Pública de Navarra-CSIC-Gobierno de NavarraPamplonaSpain

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