Binary Pattern Dictionary Learning for Gene Expression Representation in Drosophila Imaginal Discs

Conference paper
First Online:
Part of the Lecture Notes in Computer Science book series (LNCS, volume 10117)

Abstract

We present an image processing pipeline which accepts a large number of images, containing spatial expression information for thousands of genes in Drosophila imaginal discs. We assume that the gene activations are binary and can be expressed as a union of a small set of non-overlapping spatial patterns, yielding a compact representation of the spatial activation of each gene. This lends itself well to further automatic analysis, with the hope of discovering new biological relationships. Traditionally, the images were labeled manually, which was very time consuming. The key part of our work is a binary pattern dictionary learning algorithm, that takes a set of binary images and determines a set of patterns, which can be used to represent the input images with a small error. We also describe the preprocessing phase, where input images are segmented to recover the activation images and spatially aligned to a common reference. We compare binary pattern dictionary learning to existing alternative methods on synthetic data and also show results of the algorithm on real microscopy images of the Drosophila imaginal discs.

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Notes

Acknowledgement

This work was supported by the Czech Science Foundation project 14-21421S and by the Grant Agency of the Czech Technical University in Prague under the grant SGS15/154/OHK3/2T/13.

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Center for Machine Perception, Department of Cybernetics, Faculty of Electrical EngineeringCzech Technical University in PraguePragueCzech Republic

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