Approaches to Detecting Cyclospora cayetanensis: From Diagnostics to Detection in Different Food Matrices

  • Ynés R. Ortega
  • Lucy J. Robertson
Part of the SpringerBriefs in Food, Health, and Nutrition book series (BRIEFSFOOD)


Detection of Cyclospora oocysts in feces is the gold standard of clinical diagnostics, although efficient multiplex diagnostic kits relying on molecular techniques are adding to the armory. However, in order to be able to link cases, confirm transmission vehicles (often implicated epidemiologically, but sometimes with relatively flimsy evidence), and determine routes from source to infection, we need also to be able to analyze environmental matrices, particularly water samples and food, for Cyclospora. Our detection tools, be they based on molecular techniques or microscopy, are relatively efficient down to low numbers, but there is still a challenge in obtaining sufficiently clean (free of inhibitors) and concentrated samples from the original material to be able to apply the detection methodologies. Thus, efficient removal of the oocysts from food samples using elution solutions, followed by concentration procedures for subsequent detection assays should be areas of focus. In addition, in order to ensure sufficient robustness in terms of sensitivity and specificity, methods developed should be validated in inter-laboratory studies. Collaboration and sharing of material and methods is likely to be of benefit in improving our current analytical techniques.


Fresh Produce Polymerase Chain Reaction Detection Cryptosporidium Oocyst Oocyst Wall Oocyst Excretion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© The Author(s) 2017

Authors and Affiliations

  • Ynés R. Ortega
    • 1
  • Lucy J. Robertson
    • 2
  1. 1.Center for Food SafetyUniversity of GeorgiaGriffinUSA
  2. 2.Parasitology, Faculty of Veterinary MedicineNorwegian University of Life SciencesOsloNorway

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