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The Human T Cell Priming Assay (hTCPA)

  • Philipp R. Esser
  • Stefan F. Martin
Chapter

Abstract

The induction of allergic contact dermatitis by chemicals depends on their ability to activate a chemical-specific T cell response. This key event that concludes the skin sensitization process is monitored in an in vitro assay, the human T cell priming assay (hTCPA). Naive human T cells are incubated with autologous dendritic cells pulsed with the chemical of interest. After restimulation with the same chemical, the induction of an antigen-specific T cell response can be assessed by the detection of T cell proliferation, cytokine production or surface marker expression. The hTCPA still needs optimization but may nevertheless be a valuable in vitro assay within an integrated testing strategy aiming at replacement of animal testing for contact allergen identification.

Keywords

Chemical Contact allergen T cell Skin In vitro assay Animal testing 

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Allergy Research Group, Department of Dermatology, Medical Center-University of FreiburgFaculty of Medicine, University of FreiburgFreiburgGermany

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