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The epiCS® Skin Corrosion Test Method

  • Oliver Engelking
  • Dirk Weisensee
  • Horst W. Fuchs
Chapter

Abstract

The epiCS® skin corrosion test method classifying chemicals either as corrosive (category 1) or non-corrosive (no category) was validated in 2009 according to OECD TG 431. Recently, the EU Classification, Labelling and Packaging Regulation (EU CLP) system required the subcategorization of corrosive chemicals into the UN GHS optional subcategories 1A and 1B and 1C. Eighty chemicals including solids, semi-solids and liquids of different chemical classes (e.g. electrophiles, organic bases and acids, neutral organics, surfactants, inorganic salts, acids, phenols), selected by the OECD expert group on skin corrosion, were tested in two independent runs. Freeze-inactivated tissues were used to correct for direct MTT reduction and interference by colouring agents. The results with the epiCS® test method demonstrate correctly classified corrosive and non-corrosive chemicals with high sensitivity (87, 50%) and specificity (71, 62%). The overall accuracy regarding subcategorization into UN GHS subcategories 1A and 1B and 1C is 69, 81% [1]. The results demonstrate that the epiCS® method is able to correctly identify corrosive and non-corrosive chemicals and can discriminate 1A and 1B and 1C chemical categories.

References

  1. 1.
    OECD. Test Guideline (Nr. 431) for the testing of chemicals – In vitro skin Corrosion: reconstructed human epidermis (RHE) test method. 2016.Google Scholar
  2. 2.
    Hoffmann J, Heisler E, Karpinski S, Losse J, Thomas D, Siefken W, Ahr HJ, Vohr HW, Fuchs HW. Epidermal-skin-test 1000 (EST-1000)—a new reconstructed epidermis for in vitro skin corrosivity testing. Toxicol In Vitro. 2005;19:925–9.CrossRefPubMedGoogle Scholar
  3. 3.
    UN. United Nations globally harmonized system of classification and Labelling of chemicals (GHS), 6h revised ed. New York and Geneva: UN; 2015. Available at: http://www.unece.org/trans/danger/publi/ghs/ghs_rev06/06files_e.html.
  4. 4.
    Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55–63.CrossRefGoogle Scholar
  5. 5.
    ESAC. Peer review panel consensus report on the EST1000 in vitro test method for assessing skin corrosion in vitro. 2009.Google Scholar
  6. 6.
    OECD. Summary document on the statistical performance of methods in OECD test guideline 431 for sub-categorisation, OECD Environment, Health and safety Publications (EHS), series on testing and assessment, no. 190. Paris: OECD Publications; 2013.Google Scholar
  7. 7.
    Desprez B, Barroso J, Griesinger C, Kandárová H, Alépée N, Fuchs HW. Two novel prediction models improve predictions of skin corrosive sub-categories by test methods of OECD test guideline no. 431. Toxicol In Vitro. 2015;29:2055–80.CrossRefPubMedGoogle Scholar
  8. 8.
    epiCS® SOP. Version 7.0. In Vitro Skin corrosion: human skin model test epidermal skin test 1000 (epiCS®) including sub-categorization of corrosive chemicals with epiCS. CellSystems, 2017. Available at: http://www.skininvitro.com.

Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Oliver Engelking
    • 1
  • Dirk Weisensee
    • 1
  • Horst W. Fuchs
    • 1
  1. 1.CellSystems GmbHTroisdorfGermany

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