Myosin and Actin Filaments in Muscle: Structures and Interactions

  • John M. SquireEmail author
  • Danielle M. Paul
  • Edward P. Morris
Part of the Subcellular Biochemistry book series (SCBI, volume 82)


In the last decade, improvements in electron microscopy and image processing have permitted significantly higher resolutions to be achieved (sometimes <1 nm) when studying isolated actin and myosin filaments. In the case of actin filaments the changing structure when troponin binds calcium ions can be followed using electron microscopy and single particle analysis to reveal what happens on each of the seven non-equivalent pseudo-repeats of the tropomyosin α-helical coiled-coil. In the case of the known family of myosin filaments not only are the myosin head arrangements under relaxing conditions being defined, but the latest analysis, also using single particle methods, is starting to reveal the way that the α-helical coiled-coil myosin rods are packed to give the filament backbones.


Muscle thick filaments Titin Tropomyosin Myosin binding protein-C C-protein Myosin rod packing Paramyosin Troponin Nebulin 



Much of our work reported here has been funded by the British Heart Foundation, with earlier work supported by the UK Medical Research Council and the Wellcome Trust. We are indebted to all these funding agencies. JMS and DP are currently funded on the BHF Fellowship grant (FS/14/18/3071). EM is funded by Cancer Research UK (grant C12209/A16749). We are indebted to Dr. John Wray for helpful comments about crustacean myosin filaments.


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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • John M. Squire
    • 1
    Email author
  • Danielle M. Paul
    • 1
  • Edward P. Morris
    • 2
  1. 1.Muscle Contraction Group, School of Physiology, Pharmacology and NeuroscienceUniversity of BristolBristolUK
  2. 2.Division of Structural BiologyThe Institute of Cancer ResearchLondonUK

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