Measuring Process Dynamics and Nuclear Migration for Clones of Neural Progenitor Cells

  • Edgar Cardenas De La Hoz
  • Mark R. Winter
  • Maria Apostolopoulou
  • Sally Temple
  • Andrew R. CohenEmail author
Conference paper
Part of the Lecture Notes in Computer Science book series (LNCS, volume 9913)


Neural stem and progenitor cells (NPCs) generate processes that extend from the cell body in a dynamic manner. The NPC nucleus migrates along these processes with patterns believed to be tightly coupled to mechanisms of cell cycle regulation and cell fate determination. Here, we describe a new segmentation and tracking approach that allows NPC processes and nuclei to be reliably tracked across multiple rounds of cell division in phase-contrast microscopy images. Results are presented for mouse adult and embryonic NPCs from hundreds of clones, or lineage trees, containing tens of thousands of cells and millions of segmentations. New visualization approaches allow the NPC nuclear and process features to be effectively visualized for an entire clone. Significant differences in process and nuclear dynamics were found among type A and type C adult NPCs, and also between embryonic NPCs cultured from the anterior and posterior cerebral cortex.


Neural stem cells Neural progenitor cells Stem cell processes Segmentation Tracking Lineaging Stem cell process dynamics Interkinetic nuclear migration 



Portions of this research were supported by the NIH NINDS (R01NS076709), and by the NIH NIA (R01AG041861).


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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Edgar Cardenas De La Hoz
    • 1
  • Mark R. Winter
    • 1
  • Maria Apostolopoulou
    • 2
  • Sally Temple
    • 2
  • Andrew R. Cohen
    • 1
    Email author
  1. 1.Department of Electrical and Computer EngineeringDrexel UniversityPhiladelphiaUSA
  2. 2.Neural Stem Cell InstituteRensselaerUSA

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