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Novel Technology for Enrichment of Biomolecules from Cell-Free Body Fluids and Subsequent DNA Sizing

  • Vipulkumar Patel
  • Peter Celec
  • Magdalena Grunt
  • Heidi Schwarzenbach
  • Ingo Jenneckens
  • Timo HillebrandEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 924)

Abstract

Circulating cell-free DNA (ccfDNA) is a promising diagnostic tool and its size fractionation is of interest. However, kits for isolation of ccfDNA available on the market are designed for small volumes hence processing large sample volumes is laborious. We have tested a new method that enables enrichment of ccfDNA from large volumes of plasma and subsequently allows size-fractionation of isolated ccfDNA into two fractions with individually established cut-off levels of ccfDNA length. This method allows isolation of low-abundant DNA as well as separation of long and short DNA molecules. This procedure may be important e.g., in prenatal diagnostics and cancer research that have been already confirmed by our primary experiments. Here, we report the results of selective separation of 200- and 500-bp long synthetic DNA fragments spiked in plasma samples. Furthermore, we size-fractionated ccfDNA from the plasma of pregnant women and verified the prevalence of fetal ccfDNA in all fractions.

Keywords

Cell-free DNA DNA size-fractionation Prenatal diagnostics Cancer research 

Notes

Conflict of Interests

No potential conflicts of interest were disclosed.

References

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Vipulkumar Patel
    • 1
  • Peter Celec
    • 2
  • Magdalena Grunt
    • 1
  • Heidi Schwarzenbach
    • 3
  • Ingo Jenneckens
    • 4
  • Timo Hillebrand
    • 1
    Email author
  1. 1.AJ Innuscreen GmbHBerlinGermany
  2. 2.Institute of Molecular BiomedicineComenius University in BratislavaBratislavaSlovakia
  3. 3.Department of Tumor BiologyUniversity Medical Center Hamburg-EppendorfHamburgGermany
  4. 4.Analytik JenaJenaGermany

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