Whole Mastectomy Volume Reconstruction from 2D Radiographs and Its Mapping to Histology
Women that are diagnosed with breast cancer often undergo surgery to remove either the tumour and some of the surrounding tissue (lumpectomy) or the whole breast (mastectomy). After surgery, the excised tissue is sliced at the pathology department, where specimen radiographs of the slices are typically acquired. Representative parts of the tissue are then sampled for further processing, staining and examination under the microscope. The results of histopathological imaging are used for tumour characterisation. As the 3D structure of the specimen is inevitably lost during specimen handling, reconstructing a volume from individual specimen slices could facilitate the correlation of histology to radiological imaging. This work proposes a novel method for a whole specimen volume reconstruction and is validated on six mastectomy cases. We also demonstrate how these volumes can be used as a means to map multiple histology slides to a whole mastectomy image (MRI or CT).
Keywords3D volume reconstruction Breast histology-radiology registration
This study was funded by the European 7th Framework Program grant VPH-PRISM (FP7-ICT-2011-9, 601040) and the Engineering and Physical Sciences Research Council grant MIMIC (EP/K020439/1).
- 3.Alic, L., Haeck, J.C., Bol, K., Klein, S., van Tiel, S.T., Wielepolski, P.A., de Jong, M., Niessen, W.J., Bernsen, M., Veenland, J.F.: Facilitating tumor functional assessment by spatially relating 3D tumor histology and in vivo MRI: image registration approach. PloS ONE 6(8), e22835 (2011)CrossRefGoogle Scholar
- 5.Yushkevich, P.A., Avants, B.B., Ng, L., Hawrylycz, M., Burstein, P.D., Zhang, H., Gee, J.C.: 3D mouse brain reconstruction from histology using a coarse-to-fine approach. In: Pluim, J.P.W., Likar, B., Gerritsen, F.A. (eds.) WBIR 2006. LNCS, vol. 4057, pp. 230–237. Springer, Heidelberg (2006)CrossRefGoogle Scholar
- 11.Reis, S., Eiben, B., Mertzanidou, T., Hipwell, J., Hermsen, M., van der Laak, J., Pinder, S., Bult, P., Hawkes, D.: Minimum slice spacing required to reconstruct 3D shape for serial sections of breast tissue for comparison with medical imaging. In: SPIE Medical Imaging, p. 94200K. International Society for Optics and Photonics (2015)Google Scholar
- 12.Mertzanidou, T., Hipwell, J., Dalmis, M., Platel, B., van der Laak, J., Mann, R., Karssemeijer, N., Bult, P., Hawkes, D.: Towards spatial correspondence between specimen and in-vivo breast imaging. In: Fujita, H., Hara, T., Muramatsu, C. (eds.) IWDM 2014. LNCS, vol. 8539, pp. 674–680. Springer, Heidelberg (2014)Google Scholar