Tandem Mass Spectrum Sequencing: An Alternative to Database Search Engines in Shotgun Proteomics

  • Thilo Muth
  • Erdmann Rapp
  • Frode S. Berven
  • Harald Barsnes
  • Marc Vaudel
Chapter

Abstract

Protein identification via database searches has become the gold standard in mass spectrometry based shotgun proteomics. However, as the quality of tandem mass spectra improves, direct mass spectrum sequencing gains interest as a database-independent alternative. In this chapter, the general principle of this so-called de novo sequencing is introduced along with pitfalls and challenges of the technique. The main tools available are presented with a focus on user friendly open source software which can be directly applied in everyday proteomic workflows.

Keywords

de novo identification Mass spectrum sequencing Quality control Visualization 

Abbreviations

PSM

Peptide Spectrum Match

FDR

False Discovery Rate

m/z

Mass over Charge

BLAST

Basic Local Alignment Search Tool

PTM

Post-Translational Modification

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Thilo Muth
    • 1
    • 2
  • Erdmann Rapp
    • 1
    • 2
  • Frode S. Berven
    • 3
    • 4
    • 5
  • Harald Barsnes
    • 3
  • Marc Vaudel
    • 3
  1. 1.Max Planck Institute for Dynamics of Complex Technical SystemsMagdeburgGermany
  2. 2.glyXera GmbHMagdeburgGermany
  3. 3.Proteomics Unit, Department of BiomedicineUniversity of BergenBergenNorway
  4. 4.KG Jebsen Centre for Multiple Sclerosis Research, Department of Clinical MedicineUniversity of BergenBergenNorway
  5. 5.Norwegian Multiple Sclerosis Competence Centre, Department of NeurologyHaukeland University HospitalBergenNorway

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