We observed in vivo kidney dysfunction with various ischemia times at 30, 75, 90, and 120 min using multi-modality optical imaging: optical coherence tomography (OCT), Doppler OCT (DOCT), and two-photon microscopy (TPM). We imaged the renal tubule lumens and glomerulus at several areas of each kidney before, during, and after ischemia of 5-month-old female Munich-Wistar rats. For animals with 30 and 75 min ischemia times, we observed that all areas were recovered after ischemia, that tubule lumens were re-opened and the blood flow of the glomerulus was re-established. For animals with 90 and 120 min ischemia times, we observed unrecovered areas, and that tubule lumens remained close after ischemia. TPM imaging verified the results of OCT and provided higher resolution images than OCT to visualize renal tubule lumens and glomerulus blood flow at the cellular level.
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This work is partially supported by the National Institutes of Health (NIH) grants R21AG042700 and R21DK088066. We thank our collaborator Professor Peter Andrews at the Georgetown University Medical Center.
Andrews PM et al (2002) Using tandem scanning confocal microscopy to predict the status of donor kidneys. Nephron 91(1):148–155CrossRefPubMedGoogle Scholar
Schmitt JM (1999) Optical coherence tomography (OCT): a review. IEEE J Sel Top Quant Electron 5(3):1205–1215CrossRefGoogle Scholar
Hee MR et al (1995) Optical coherence tomography of the human retina. Arch Ophthalmol 113(3):325–332, Chicago, IL, 1960CrossRefPubMedGoogle Scholar
Andrews PM et al (2008) High-resolution optical coherence tomography imaging of the living kidney. Lab Invest 88(4):441–449CrossRefPubMedGoogle Scholar
Wierwille J et al (2011) In vivo, label-free, three-dimensional quantitative imaging of kidney microcirculation using Doppler optical coherence tomography. Lab Invest 91(11):1596–1604CrossRefPubMedPubMedCentralGoogle Scholar
Chen Y et al (2013) Recent advances in two-photon imaging: technology developments and biomedical applications. Chin Opt Lett 11(1):011703CrossRefGoogle Scholar