Assessing Fungal Biodiversity Using Molecular Markers

  • Md. Shamim
  • Pankaj Kumar
  • Ravi Ranjan Kumar
  • Mahesh Kumar
  • Ranjeet Ranjan Kumar
  • K. N. Singh
Chapter
Part of the Fungal Biology book series (FUNGBIO)

Abstract

With an advent of modern molecular markers technology, it has become easier for the rapid identification, characterization and detection of important plant fungus that were previously a major bottleneck to detect by traditional microbiological methods. Present molecular tools in fungal biodiversity can have potential to characterize in very rapid manner by small quantity of DNA/RNA of fungal pathogens in short period. Different molecular markers viz.; RFLP, RAPD, SSR, AFLP, ITS, IGS and other markers have accelerated the identification and characterization of important plant fungus since previous decades. Internal transcribed spacer (ITS) region of ribosomal RNA genes is widely accepted technique for explanation of the diversity among fungal communities due to its utility for the identification of fungi to genus/ species taxonomic levels, due to its several important including its high representation in public sequence databases. Investigation of fungal diversity has also promoted since introduction of next-generation DNA sequencing technologies. Presently, high-throughput sequencing techniques are widely adopted by the several researchers for the detection of biodiversity of fungal populations. At present, construction of sequence databases of different fungal species that have broaden the representation across fungal population by bioinformatics and taxonomic experts is a critical next step towards the assessments of fungal diversity.

Keywords

Internal Transcribe Spacer Amplify Fragment Length Polymorphism Simple Sequence Repeat Marker Inter Simple Sequence Repeat Amplify Fragment Length Polymorphism Marker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer International Publishing Switzerland 2017

Authors and Affiliations

  • Md. Shamim
    • 1
    • 2
  • Pankaj Kumar
    • 2
  • Ravi Ranjan Kumar
    • 2
  • Mahesh Kumar
    • 2
  • Ranjeet Ranjan Kumar
    • 3
  • K. N. Singh
    • 1
  1. 1.Department of Plant Molecular Biology and Genetic EngineeringN.D. University of Agriculture and TechnologyKumarganj, FaizabadIndia
  2. 2.Department of Molecular Biology and Genetic EngineeringBihar Agricultural UniversitySabour, BhagalpurIndia
  3. 3.Division of BiochemistryIndian Agricultural Research InstitutePusaIndia

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