Electroporation-Enhanced Transdermal Delivery of Patent Blue Using Green Skin Pore Device
Electroporation was used as an enhancement method for transdermal delivery of a model molecule, Patent Blue. The level of increase of stratum corneum permeability was assessed with a numerical model and experimentally on ex vivo full-thickness porcine ear skin. Green Skin Pore square wave pulse generator (Iskra Medical LLC, Slovenia) was used in the study. The pulses were delivered with an array of 7 pin electrodes arranged in a honeycomb configuration. Our results show increased delivery of Patent blue after pretreatment of skin with electric pulses. Also, the extent of the permeabilized stratum corneum is higher and more homogeneous when the electrode is moved in circular motion during pulse delivery. In this way, larger portion of the skin under the electrode is exposed to electric pulses, leading to increased efficiency.
KeywordsTransdermal molecular delivery Skin electroporation Patent blue Numerical model Ex vivo histological analysis
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