Proteomic Network Systems Analysis

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Abstract

Proteomics and other high throughput technologies generate extensive molecular lists, the scope of which renders their accurate interpretation a daunting task. Thus, generalizable approaches by which to extract insight from this complexity are indispensable. Network systems biology principles and their application offer a modular, interchangeable data analytics pipeline by which to collate, integrate, and prioritize such datasets. By understanding the basis and utility of various organizing and interpretive profiling elements including ontological classification, functional enrichment and over-representation algorithms, and combining these with pathway analysis resources and the versatile tools and applications of complex network analysis, an applied network systems approach yields actionable insights into tackling the biology underlying high throughput data. Providing a framework to proteomic newcomers and experienced practitioners alike, we here outline data analytic approaches and provide concrete examples of the pairing of network systems prognostication with informed follow-up, through application of complementary physiological experimentation to validate proteomic observations in cardiovascular health and disease.

Keywords

Bioinformatics Cardiac Cardiovascular Complex network analysis Heart disease Network biology Protein Proteome Systems biology 
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Notes

Acknowledgements

Dr. Arrell is supported by a Ruth L. Kirschstein National Research Service Award from the National Institutes of Health. Dr. Terzic holds the Marriott Family Professorship in Cardiovascular Research and is the Michael S. and Mary Sue Shannon Director, Mayo Clinic Center for Regenerative Medicine. This work was supported by the National Institutes of Health (T32HL07111), Fondation Leducq, Marriott Heart Disease Research Program, and Mayo Clinic Center for Regenerative Medicine.

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Center for Regenerative Medicine and Marriott Heart Disease Research Program, Division of Cardiovascular Diseases, Department of Medicine, Department of Molecular Pharmacology and Experimental Therapeutics, and Department of Medical GeneticsMayo ClinicRochesterUSA

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