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Current Status of Microfluidics-Assisted Cytology: The Application in Molecular Cytology

  • Oladunni Adeyiga
  • Albert J. Mach
  • Jianyu Rao
  • Dino Di Carlo
Chapter
Part of the Essentials in Cytopathology book series (EICP, volume 26)

Abstract

Recent advances in miniaturization and fluid handling enabled by microfluidics are poised to have an impact in the preparation of clinical samples and analysis of cells. In this book chapter we introduce the fundamental technological advances and microfluidic technologies that address current challenges in cytopathology. We illustrate how these technologies can expand the opportunities for analysis and samples made available for use by the cytopathologist.

For example, by isolating rare subpopulations of cells from blood or expeditiously purifying body fluid samples, these technologies can improve cytomorphological, molecular, and cytogenetic testing by the cytopathologist. Contrary to previous smear-based conventional cytology and current liquid-based cytology preparation methods, microfluidics-assisted cytology techniques may provide more suitable cell preparations for molecular analysis. Many of these technologies are currently in development in academic labs; however, commercialization is in progress, and microfluidics-assisted cytology promises to reach the clinical lab in the near future.

Keywords

Microfluidics Microfabrication Soft lithography Photoresist Polydimethylsiloxane Centrifuge-on-a-Chip 

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Oladunni Adeyiga
    • 1
    • 2
    • 3
  • Albert J. Mach
    • 4
  • Jianyu Rao
    • 5
  • Dino Di Carlo
    • 2
    • 3
    • 6
    • 7
  1. 1.Division of Infectious Diseases, Department of MedicineDavid Geffen School of Medicine at University of California, Los AngelesLos AngelesUSA
  2. 2.Department of BioengineeringHenry Samueli School of Engineering & Applied Science at University of California, Los AngelesLos AngelesUSA
  3. 3.Nanoelectronics Research FacilityUniversity of California, Los AngelesLos AngelesUSA
  4. 4.Becton, Dickinson and CompanyFranklin LakesUSA
  5. 5.Department of Pathology and Laboratory MedicineUniversity of California at Los AngelesLos AngelesUSA
  6. 6.California NanoSystems Institute at University of California, Los AngelesLos AngelesUSA
  7. 7.Jonsson Comprehensive Cancer Center at University of California, Los AngelesLos AngelesUSA

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