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Automation of Technology for Cancer Research

  • Wietske van der Ent
  • Wouter J. Veneman
  • Arwin Groenewoud
  • Lanpeng Chen
  • Claudia Tulotta
  • Pancras C. W. Hogendoorn
  • Herman. P. SpainkEmail author
  • B. Ewa Snaar-JagalskaEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 916)

Abstract

Zebrafish embryos can be obtained for research purposes in large numbers at low cost and embryos develop externally in limited space, making them highly suitable for high-throughput cancer studies and drug screens. Non-invasive live imaging of various processes within the larvae is possible due to their transparency during development, and a multitude of available fluorescent transgenic reporter lines.

To perform high-throughput studies, handling large amounts of embryos and larvae is required. With such high number of individuals, even minute tasks may become time-consuming and arduous. In this chapter, an overview is given of the developments in the automation of various steps of large scale zebrafish cancer research for discovering important cancer pathways and drugs for the treatment of human disease. The focus lies on various tools developed for cancer cell implantation, embryo handling and sorting, microfluidic systems for imaging and drug treatment, and image acquisition and analysis. Examples will be given of employment of these technologies within the fields of toxicology research and cancer research.

Keywords

Zebrafish Cancer Automation High-throughput Robotics Microfluidics Image analysis 

Notes

Acknowledgements

This work was supported by Stichting Kinderen Kankervrij, Project 30677

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Wietske van der Ent
    • 1
    • 2
  • Wouter J. Veneman
    • 1
  • Arwin Groenewoud
    • 1
  • Lanpeng Chen
    • 1
  • Claudia Tulotta
    • 1
  • Pancras C. W. Hogendoorn
    • 2
  • Herman. P. Spaink
    • 1
    Email author
  • B. Ewa Snaar-Jagalska
    • 1
    Email author
  1. 1.Institute of BiologyLeiden UniversityLeidenThe Netherlands
  2. 2.Department of PathologyLeiden University Medical CenterLeidenThe Netherlands

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