Phytochemical Screening and HPLC Analysis of Artemisia amygdalina
Abstract
This chapter deals with the qualitative analysis of wild and tissue culture raised regenerants of Artemisia amygdalina, for the amount of bioactive principles particularly the antimalarial compound, artemisinin. Phytochemical screening of extracts revealed the presence of terpenes, alkaloids, phenolics, tannins (polyphenolics), cardiac glycosides, and steroids in wild (aerial, inflorescence) and tissue culture regenerants (in vitro grown plant, callus,and greenhouse acclimatized plants). Further, HPLC of A. amygdalina extracts has revealed the presence of artemisinin in petroleum ether extracts of wild aerial part, tissue culture raised plant, and greenhouse acclimatized plants. Acetonitrile and water in 70:30 ratios at a flow rate of 1 ml/min have been optimized as mobile phase. It has been observed that wild inflorescences and callus do not produce artemisinin.
Keywords
Qualitative analysis HPLC Artemisinin Tissue culture Regenerants Wild plantsReferences
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